Development of a novel fluorescent microsphere technique to combine serial cerebral blood flow measurements with histology in the rat
Development of a novel fluorescent microsphere technique to combine serial cerebral blood flow measurements with histology in the rat
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DOI：
10.1016/s0165-0270(02)00316-3
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发表时间：
2003-01-30
影响因子：
3
通讯作者：
van Rossem, K
中科院分区：
文献类型：
--
作者：
De Visscher, G;Haseldonckx, M;van Rossem, K
本研究的目的是评价微球技术在同一动物不同时间点区域脑血流量（rCBF）定量评估中的应用。将直径为15 μ m的黄绿色和红色荧光微球通过导管插入左心室，在两个不同的时间点注射到大鼠体内。研究了在正碳酸条件下rCBF测量的可重复性（n = 7）和7% CO2诱导的高碳酸血症（n = 7）下血流的响应性。在100个灌注固定脑的母振动体切片上计数荧光球，计算rCBF。第一次微球注射的中位总CBF为224 ml/min/100 g，第二次微球注射的中位总CBF为216 ml/min/100 g。高碳酸组CBF为400 ml/min/100 g，正常碳酸30 min后CBF降至178 ml/min/100 g。左半球和右半球之间没有发现差异，也没有迹象表明第一次注射可能会影响第二个半球。所描述的方法可以将生理或病理条件下不同时间点的rCBF评估与同一动物大脑同一区域相关形态学改变的组织学评估相结合。(C) 2002 Elsevier Science B.V.版权所有
The aim of the study was to evaluate the microsphere technique for the quantitative assessment of regional cerebral blood flow (rCBF) at different time points in the same animal. Yellow-green and red fluorescent microspheres with a diameter of 15 mum were injected into the rat at two different time points via a cannula inserted into the left ventricle of the heart. The reproducibility of the rCBF measurements in normocapnic conditions (n = 7) and the responsiveness of the flow to hypercapnia induced by 7% CO2 (n = 7) was examined. The fluorescent spheres were counted on 100 mum vibratome sections of perfusion-fixed brains and rCBF was calculated. The median total CBF in normocapnic rats was 224 ml/min/100 g for the first microsphere injection and 216 ml/min/100 g for the second one. In the hypercapnic group CBF amounted to 400 ml/min/100 g and after 30 min of normocapnia decreased to 178 ml/min/100 g. No differences between the left and right hemisphere were found and there was no indication that the first injection might have influenced the second one. The described approach allows combining the assessment of rCBF at different time points in physiological or pathological conditions with histological evaluation of related morphological alterations in the same brain region of the same animal. (C) 2002 Elsevier Science B.V. All rights reserved.