Intracellular free calcium in rat anterior pituitary cells monitored by fura-2.
Intracellular free calcium in rat anterior pituitary cells monitored by fura-2.
复制标题
通过fura-2监测大鼠垂体前叶细胞的细胞内游离钙。
DOI：
10.1016/0024-3205(87)90230-x
复制
发表时间：
1987
期刊：
影响因子：
6.1
通讯作者：
Cronin,MJ
中科院分区：
文献类型：
--
作者：
Anderson,JM;Yasumoto,T;Cronin,MJ
大鼠垂体前叶细胞，加载与钙指示染料Fura-2后，原代培养，挑战催乳素和生长激素促分泌素和抑制激素。为了初步验证该技术，钙通道激活剂maitotoxin有效地增加细胞内游离钙（[Ca++]。不同浓度促甲状腺激素释放激素或血管紧张素II诱导的[Ca++]i峰值增加在15秒内，其次是较低和延长的平台期。抑制激素多巴胺和生长抑素最大限度地减少[Ca++]i的15-20秒，随后自发返回到基线超过5-10分钟。受体拮抗剂saralacin和spiperone阻断血管紧张素II和多巴胺的作用，分别。因此，fura-2似乎是一种足够的探针，可以解决垂体内部细胞激素受体激活诱导的[Ca++]i的秒到秒变化。
Rat anterior pituitary cells, loaded with the calcium indicator dye fura-2 after primary culture, were challenged with prolactin and growth hormone secretagogues and inhibitory hormones. To initially validate the technique, the calcium channel activator maitotoxin effectively increased intracellular free calcium ([Ca++]. Various concentrations of the secretagogues thyrotropin releasing hormone or angiotensin II induced peak increases in [Ca++]i within 15 sec, followed by a lower and prolonged plateau phase. The inhibitory hormones dopamine and somatostatin maximally reduced [Ca++]i by 15–20 sec, followed by a spontaneous return to baseline over 5–10 min. The receptor antagonists saralacin and spiperone blocked the angiotensin II and dopamine effects, respectively. Thus, fura-2 appears to be an adequate probe for resolving second-to-second changes in [Ca++]i induced by hormone receptor activation in interior pituitary cells.