Prevalence of serum antibodies against Saprolegnia parasitica in wild and farmed brown trout Salmo trutta
Prevalence of serum antibodies against Saprolegnia parasitica in wild and farmed brown trout Salmo trutta
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DOI：
10.3354/dao01999
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发表时间：
2009-01-28
影响因子：
1.4
通讯作者：
Aller-Gancedo, J. M.
中科院分区：
文献类型：
--
作者：
Fregeneda-Grandes, J. M.;Carbajal-Gonzalez, M. T.;Aller-Gancedo, J. M.
采用酶联免疫吸附试验（ELISA）研究了西班牙莱昂省野生和养殖褐鳟鱼萨尔莫的寄生水霉血清抗体的流行情况。从健康和水霉感染的褐鳟鱼的血液样本收集超过2年的季节性周期（1月，4月，7月和10月），从孵化场和河流水霉病（波马河）的频繁存在，并从河流中，很少观察到疾病（奥马哈河）。个体患病率为30.1%，但孵化场鳟鱼的患病率之间存在统计学显著差异（43.0%），来自波马河（31.8%）和奥马哈河（6.4%），10月观察到的患病率（42.9%）与1月（24.8%）、4月（22.7%）和7月（27.5%）获得的值之间也存在差异。雌鱼（34.8%）和雄鱼（38.2%）的血清阳性率没有差异，但血清抗体水平的升高与鱼体的较大（年龄较大）呈正相关。在水霉感染的鳟鱼中观察到的抗体的低流行率（18.0%）表明可能的免疫抑制和缺乏有效的特异性免疫应答。
The prevalence of serum antibodies against Saprolegnia parasitica in wild and farmed brown trout Salmo trutta from the province of Leon (NW Spain) was studied by enzyme-linked immunosorbent assay (ELISA). Blood samples from healthy and Saprolegnia-infected brown trout were collected over 2 yr with a seasonal periodicity (January, April, July and October) from a hatchery and river with frequent presence of saprolegniosis (River Porma) and from a river in which the disease was rarely observed (River Omaha). The individual prevalence was 30.1 %, but statistically significant differences were observed between the prevalence in trout from the hatchery (43.0%), from River Porma (31.8 %) and from River Omaha (6.4 %) and also between the prevalence observed in October (42.9 %) and the values obtained in January (24.8 %), April (22.7 %) and July (27.5 %). There was no difference between the seroprevalence in females (34.8 %) and males (38.2 %), but a positive correlation between raised serum antibody levels and larger (older) fish was found. The low prevalence of antibodies observed in Saprolegnia-infected trout (18.0%) suggests possible immune suppression and the lack of an effective specific immune response in fish with saprolegniosis.