ANTIGEN-REACTIVE T-CELL CLONES .1. TRANS-COMPLEMENTING HYBRID I-A-REGION GENE-PRODUCTS FUNCTION EFFECTIVELY IN ANTIGEN PRESENTATION
ANTIGEN-REACTIVE T-CELL CLONES .1. TRANS-COMPLEMENTING HYBRID I-A-REGION GENE-PRODUCTS FUNCTION EFFECTIVELY IN ANTIGEN PRESENTATION
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DOI：
10.1084/jem.152.4.759
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发表时间：
1980-01-01
影响因子：
15.3
通讯作者：
FATHMAN, CG
中科院分区：
文献类型：
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作者：
KIMOTO, M;FATHMAN, CG
有可能在体外增殖抗原特异性鼠T细胞，从而特异性逐步富集抗原诱导的增殖细胞。这些T细胞的增殖反应是抗原特异性的，并且依赖于与增殖T细胞共享I-A亚区的抗原呈递细胞（脾细胞）的存在。使用软琼脂克隆技术，它已经有可能从这样的长期培养分离抗原反应性T淋巴细胞的克隆。表明这些是抗原反应性T细胞克隆的数据是通过用一组这样的抗原反应性T细胞克隆研究与抗原呈递细胞相关的抗原的识别而获得的。通过亚克隆获得克隆性的证明。来自F1免疫小鼠的克隆可分为以下3个单独的类别：一个克隆识别与亲本A和F1的抗原呈递决定簇相关的抗原;第2种类型识别与亲本B和F1的抗原呈递决定簇相关的抗原;第3种类型仅识别与F1小鼠的抗原呈递决定簇相关的抗原。对主要组织相容性复合体向这种F1反应性T细胞克隆呈递抗原的要求的遗传学研究表明，该系统中的杂合抗原呈递决定子来自单倍型a和B的I-A区产物的反式互补。在（A/J × A）上存在唯一的F1杂种决定簇的概念是：支持C57 BL/6）F1 [小鼠]细胞;这些决定簇可在免疫活性细胞相互作用中被杂交小鼠生理利用。
It is possible to propagate antigen-specific murine T cells in vitro with resultant specific stepwise enrichment of antigen-induced proliferative cells. The proliferative responses of these T cells are antigen specific and dependent upon the presence of antigen-presenting cells (spleen cells) that share the I-A subregion with the proliferating T cell. Using techniques of soft-agar cloning, it has been possible to isolate clones of antigen-reactive T lymphocytes from such long-term cultures. Data suggesting that these were clones of antigen-reactive T cells were obtained by studying the recognition of antigen in association with antigen-presenting cells with a panel of such clones of antigen-reactive T cells. Proof of clonality was obtained by subcloning. Clones derived from F1-immune mice can be divided into the following 3 separate categories: one clone recognizes antigen in association with antigen-presenting determinants of parent A and the F1; the 2nd type recognizes antigen in association with antigen-presenting determinants of parent B and the F1; the 3rd type recognizes antigen only in association with antigen-presenting determinants of the F1 mouse. Genetic studies on the major histocompatibility complex requirements for antigen presentation to such F1-reactive T cell clones suggests that the hybrid antigen-presenting determinnant in this system results from transcomplementation of products of the I-A region of haplotypes a and b. The concept that there exist unique F1 hybrid determinants on (A/J .times. C57BL/6)F1 [mouse] cells was supported; these determinants can be utilized physiologically by hybrid mice in immunocompetent cellular interactions.