The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast
The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast
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DOI：
10.1073/pnas.96.4.1480
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发表时间：
1999-02-16
影响因子：
11.1
通讯作者：
Fink, GR
中科院分区：
文献类型：
--
作者：
Gaxiola, RA;Rao, R;Fink, GR
拟南芥液泡H+-焦磷酸酶（AVP 1）的过表达赋予盐敏感的酿酒酵母ena 1突变体的耐盐性。抑制盐敏感性需要两个离子转运蛋白，Gef 1 Cl-通道和Nhx 1 Na+/H+交换器。这两种蛋白共定位于酵母的前液泡区室，并且被认为是该区室的最佳酸化所需的。AtNHX 1（酵母Na+/H+交换器的植物同源物）的过表达抑制了酵母nhx 1突变体的一些突变表型。此外，拟南芥AtNHX 1 mRNA的水平在NaCl的存在下增加。AtNHX 1的NaCl和植物基因的能力，以抑制酵母nHX 1突变体的调节表明，阳离子在酵母和植物中解毒的机制可能是相似的。
Overexpression of the Arabidopsis thaliana vacuolar H+-pyrophosphatase (AVP1) confers salt tolerance to the salt-sensitive ena1 mutant of Saccharomyces cerevisiae. Suppression of salt sensitivity requires two ion transporters, the Gef1 Cl- channel and the Nhx1 Na+/H+ exchanger. These two proteins colocalize to the prevacuolar compartment of yeast and are thought to be required for optimal acidification of this compartment, Overexpression of AtNHX1, the plant homologue of the yeast Na+/H+ exchanger, suppresses some of the mutant phenotypes of the yeast nhx1 mutant. Moreover, the level of AtNHX1 mRNA in Arabidopsis is increased in the presence of NaCl. The regulation of AtNHX1 by NaCl and the ability of the plant gene to suppress the yeast nhx1 mutant suggest that the mechanism by which cations are detoxified in yeast and plants may be similar.