G and P genotypes of group A rotavirus strains circulating in calves in Brazil, 1996-1999
G and P genotypes of group A rotavirus strains circulating in calves in Brazil, 1996-1999
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DOI：
10.1016/j.vetmic.2003.10.029
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发表时间：
2004-04-19
影响因子：
3.3
通讯作者：
Richtzenhain, LJ
中科院分区：
文献类型：
--
作者：
Alferi, AF;Alfieri, AA;Richtzenhain, LJ
采用巢式多重逆转录/聚合酶链反应（Nested multiplex/RT-PCR）对50份A组轮状病毒（RV）酶免疫分析（EIA）和聚丙烯酰胺凝胶电泳（PAGE）均阳性的腹泻犊牛粪便样品进行了P和G基因型鉴定。1996年至1999年期间，从位于巴西南马托格罗索、圣保罗、戈亚斯和巴拉那州的8个奶牛和/或肉牛群中采集了样本。在44份（88%）小牛粪便样本中可以进行完整的基因分型。在6份（12%）样本中无法识别VP 7基因。而VP 4（P）基因在100%的样品中被鉴定。35份（70%）样品的基因型对应于NCDV-like（12%）、UK-like（40%）、B223-like（16%）和KN-4（2%）参考轮状病毒株，这些参考轮状病毒株在牛中常见。混合感染7份（14%），其中2份（4%）基因型为非正常组合，分别为牛源A组轮状病毒VP 4和VP 7基因（P[11]、G8），另1份（P[1]）为牛源VP 4基因和猪源VP 7基因（G5）。(C)2004年由Elsevier B. V.出版
Fifty fecal samples from calves with diarrhea, positive for group A rotavirus by enzyme immunoassay (EIA) and polyacrylamide gel electrophoresis (PAGE) were analyzed by a nested multiplex reverse transcription/polymerase chain reaction (Nested multiplex/RT-PCR) for identification of P and G genotypes. Samples were collected between 1996 and 1999 from eight dairy and/or beef cattle herds located in the Mato Grosso do Sul, Sao Paulo, Goias and Parana States, Brazil. Complete genotyping was possible in 44 (88%) of the calf fecal samples. The VP7 gene could not be identified in six (12%) of the samples. Whilst the VP4 (P) gene was identified in 100% of samples. The genotypes of 35 (70%) samples corresponded to the NCDV-Iike (12%), UK-like (40%), B223-like (16%) and KN-4 (2%) reference rotavirus strains, which are commonly found in cattle. Mixed infections were detected in seven (14%) samples, and genotypes observed in two (4%) samples presented unusual combinations, carrying VP4 and VP7 gene of the bovine group A rotavirus (P[11],G8), in other one carrying a VP4 gene of bovine origin (P[l]) and a VP7 gene of swine origin (G5). (C) 2004 Published by Elsevier B.V.