Functional analysis of Pdp1 and vrille in the circadian system of a cricket.
Functional analysis of Pdp1 and vrille in the circadian system of a cricket.
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DOI：
10.1016/j.jinsphys.2020.104156
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发表时间：
2020-10
影响因子：
2.2
通讯作者：
Yumina Narasaki-Funo;Yasuaki Tomiyama;Motoki Nose Data Analysis;T. Bando;K. Tomioka
中科院分区：
文献类型：
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作者：
Yumina Narasaki-Funo;Yasuaki Tomiyama;Motoki Nose Data Analysis;T. Bando;K. Tomioka
昼夜节律是由生物钟产生的，而生物钟的振荡是基于所谓的时钟基因的有节奏的表达。本研究探讨了双斑蟋蟀Gb‘vri和Par结构域蛋白1(Gb’Pdp1)在蟋蟀生物钟中的作用。Gb‘vri和Gb’Pdp1cDNA的结构分析表明，它们是bZIP转录因子的成员。在光/暗周期(LD)下，这两个基因在时钟组织、视叶中有节律性表达，而在恒定黑暗(DD)下，节律性减弱。Gb‘clk和Gb’cyc的RNA干扰(RNAi)显著降低了Gb‘vri和Gb’Pdp1mRNA的表达水平，表明它们受Gb‘clk/GB’Cyc调控。Gb‘vri和Gb’Pdp1的RNAi对运动节律几乎没有影响，但当与Gb‘cycRNAi一起处理时，它们的影响变得明显。Gb‘vriRNAi/Gb’cycRNAi/Gb‘cycRNAi的平均自由运行周期显著短于Gb’cRNAi/Gb‘cycRNAi。Gb‘Pdp1RNAi/Gb’cycRNAi处理的细胞周期也有类似的缩短。一些Gb‘Pdp1RNAi/Gb’cycRNAi表现出节律分裂为两个不同时期的自由运行成分，Gb‘vriRNAi和Gb’Pdp1RNAi处理显著改变了Gb‘Clk、GB’Cyc和Gb‘timin Ld的表达。这些结果表明，Gb‘vri和Gb’Pdp1在板球生物钟中起着重要作用。
Circadian rhythms are generated by a circadian clock for which oscillations are based on the rhythmic expression of the so-called clock genes. The present study investigated the role ofGryllus bimaculatus vrille(Gb’vri) andPar domain protein 1(Gb’Pdp1) in the circadian clock of the cricketGryllus bimaculatus.Structural analysis ofGb’vriandGb’Pdp1cDNAs revealed that they are a member of the bZIP transcription factors. Under light/dark cycles (LD) both genes were rhythmically expressed in the clock tissue, the optic lobes, whereas the rhythm diminished under constant darkness (DD).Gb’vriandGb’Pdp1mRNA levels were significantly reduced by RNA interference (RNAi) ofGb’ClkandGb’cyc, suggesting they are controlled byGb’CLK/Gb’CYC. RNAi ofGb’vriandGb’Pdp1had little effect on locomotor rhythms, although their effects became visible when treated together withGb’cycRNAi. The average free-running period ofGb’vriRNAi/Gb’cycRNAicrickets was significantly shorter than that ofGb’cycRNAicrickets. A similar period shortening was observed also when treated withGb’Pdp1RNAi/Gb’cycRNAi. SomeGb’Pdp1RNAi/Gb’cycRNAicrickets showed rhythm splitting into two free-running components with different periods.Gb’vriRNAiandGb’Pdp1RNAitreatments significantly altered the expression ofGb’Clk,Gb’cyc, andGb’timin LD. These results suggest thatGb’vriandGb’Pdp1play important roles in cricket circadian clocks.