Assessment and analysis of mechanical allodynia-like behavior induced by spared nerve injury (SNI) in the mouse
Assessment and analysis of mechanical allodynia-like behavior induced by spared nerve injury (SNI) in the mouse
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DOI：
10.1016/j.pain.2005.10.036
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发表时间：
2006-05-01
期刊：
影响因子：
7.4
通讯作者：
Decosterd, Isabelle
中科院分区：
文献类型：
--
作者：
Bourquin, Anne-Frederique;Sueveges, Maria;Decosterd, Isabelle
已经开发了周围神经损伤的实验模型来研究神经性疼痛的机制。在大鼠的备用神经损伤（SNI）模型中，腓总神经和胫神经受到损伤，在备用腓肠神经区域产生一致且可重复的疼痛超敏反应。在这项研究中，我们研究了小鼠 SNI 是否也是神经性疼痛的有效模型系统。 SNI 导致 SNI 手术小鼠的戒断阈值显着降低。这种效果在动物之间非常一致，并且在研究的四个星期内持续存在。我们还确定了一系列 11 根 von Frey 毛发中每根毛发缩爪的相对频率。使用整合所有变量（神经损伤、爪子、性别和时间）的混合效应模型对响应频率进行分析，显示 SNI 随着时间的推移具有非常稳定的效果，并且还揭示了变量之间的细微差异，包括基于性别的机械敏感性差异。我们测试了 SNI 模型的两种变体，发现单独损伤胫神经会引起机械过敏，而同时损伤腓总神经和腓肠神经不会引起未受损伤的胫神经区域机械敏感性的任何显着增加。 SNI 在小鼠中诱导机械异常性疼痛样反应，我们相信我们改进的评估和数据分析方法将揭示持续性疼痛模型中额外的内部和外部变异因素。在基因改造小鼠中使用该模型对于确定神经性疼痛的机制应该非常有效。 (c) 2005 年国际疼痛研究协会。由爱思唯尔出版。 B.V.保留所有权利。
Experimental models of peripheral nerve injury have been developed to study mechanisms of neuropathic pain. In the spared nerve injury (SNI) model in rats, the common peroneal and tibial nerves are injured, producing consistent and reproducible pain hypersensitivity in the territory of the spared sural nerve. In this study, we investigated whether SNI in mice is also a valid model system for neuropathic pain. SNI results in a significant decrease in withdrawal threshold in SNI-operated mice. The effect is very consistent between animals and persists for the four weeks of the study. We also determined the relative frequency of paw withdrawal for each of a series of 11 von Frey hairs. Analysis of response frequency using a mixed-effects model that integrates all variables (nerve injury, paw, gender, and time) shows a very stable effect of SNI over time and also reveals subtle divergences between variables, including gender-based differences in mechanical sensitivity. We tested two variants of the SNI model and found that injuring the tibial nerve alone induces mechanical hypersensitivity, while injuring the common peroneal and sural nerves together does not induce any significant increase in mechanical sensitivity in the territory of the spared tibial nerve. SNI induces a mechanical allodynia-like response in mice and we believe that our improved method of assessment and data analysis will reveal additional internal and external variability factors in models of persistent pain. Use of this model in genetically altered mice should be very effective for determining the mechanisms involved in neuropathic pain. (c) 2005 International Association for the Study of Pain. Published by Elsevier. B.V. All rights reserved.