Tyrosinase gene expression in human tissues.
Tyrosinase gene expression in human tissues.
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DOI：
10.1111/j.1600-0749.1993.tb00622.x
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发表时间：
1993-12
期刊：
影响因子：
--
通讯作者：
Zit Abattyani;Luc Xerri;Jacques Hassoun;Bonérandi Jj;Jean-Jacques Grob
中科院分区：
文献类型：
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作者：
Zit Abattyani;Luc Xerri;Jacques Hassoun;Bonérandi Jj;Jean-Jacques Grob
原发性皮肤外恶性黑色素瘤（MM）的偶尔发生导致了这样的假设，即来自神经嵴的黑色素细胞可能在其迁移中被逮捕，并可能发生原位恶性转化。然而，痣细胞的聚集体很少被确定在少数器官以外的皮肤和眼睛的组织学检查。酪氨酸酶是一种黑色素生物合成酶，被认为是黑色素细胞分化的最特异性标志物之一。我们已经尝试通过酪氨酸酶基因表达检测人类组织中定向为黑素细胞谱系的细胞。从正常组织和肿瘤组织中提取总RNA，并使用高灵敏度的逆转录PCR分析，引物特异性酪氨酸酶基因。来自健康受试者的外周血单核细胞（PBMC）用作阴性对照。酪氨酸酶转录本在广泛的正常器官中被鉴定，如皮肤、淋巴结、胃窦、结肠、肾、肺、睾丸、卵巢、乳腺和外周神经。酪氨酸酶RNA也在肿瘤样本中检测到，包括良性皮肤痣、MM累及的淋巴结、乳腺癌、脂肪肉瘤、恶性淋巴瘤和神经鞘瘤。转移性MM患者的PBMC也呈阳性，而其他癌症患者的血液标本中未检测到阳性。因此，似乎表达酪氨酸酶基因的细胞存在于广泛的人体组织中。虽然这些细胞仍然必须被准确地鉴定，但可以提出它们可能对应于完全分化的黑素细胞、黑素细胞前体或具有黑素细胞分化潜力的许旺细胞。（250字处删节）
The occasional occurrence of primary extra-cutaneous malignant melanomas (MM) has led to the hypothesis that melanocytes derived from the neural crest may be arrested in their migration and may undergo an in situ malignant transformation. However, aggregates of nevus cells have only rarely been identified by histological examination in a few organs other than skin and eye. Tyrosinase is a melanin biosynthetic enzyme that is considered one of the most specific markers of melanocytic differentiation. We have attempted to detect cells committed to the melanocytic lineage, in human tissues, by means of tyrosinase gene expression. Total RNA was extracted from normal and neoplastic tissues and analyzed using a highly sensitive reverse transcription PCR assay with primers specific for the tyrosinase gene. Peripheral blood mononuclear cells (PBMC) from healthy subjects were used as negative controls. Tyrosinase transcripts were identified in a wide range of normal organs such as skin, lymph nodes, antrum, colon, kidney, lung, testis, ovary, breast, and peripheral nerve. Tyrosinase RNA was also detected in neoplastic samples including benign cutaneous nevi, lymph nodes involved by MM, breast carcinoma, liposarcoma, malignant lymphoma, and schwannoma. PBMC from patients with metastatic MM were also positive, while no positivity was detected in blood specimens from patients with other cancers. Therefore, it appears likely that cells expressing the tyrosinase gene are present in a wide range of human tissues. Although these cells still have to be accurately identified, one could propose that they might correspond to either fully differentiated melanocytes, melanocytic precursors, or Schwann cells bearing potentialities of melanocytic differentiation.(ABSTRACT TRUNCATED AT 250 WORDS)