SELECTIVE LOCALIZATION OF MESSENGER-RNA FOR CYTOSKELETAL PROTEIN MAP2 IN DENDRITES
SELECTIVE LOCALIZATION OF MESSENGER-RNA FOR CYTOSKELETAL PROTEIN MAP2 IN DENDRITES
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DOI：
10.1038/336674a0
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发表时间：
1988-12-15
期刊：
影响因子：
64.8
通讯作者：
MATUS, A
中科院分区：
文献类型：
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作者：
GARNER, CC;TUCKER, RP;MATUS, A
为了使神经细胞形成高度极化的形式，必须将适当的结构分子靶向轴突或树突。这可以通过在细胞体中合成结构蛋白并通过特定的运输机制将其分选到轴突或树突来实现。对于树突，另一种可能性是蛋白质可以在树突细胞质中局部合成。这是一个有吸引力的想法，因为它将允许调节结构分子的产生，以响应树突发育期间的局部需求。树突状蛋白质合成的可行性，建议树突状多核糖体的存在1和最近的证明，新合成的RNA被运送到树突的神经元分化的文化2。然而，到目前为止，还没有证明在树突细胞质中选择性合成鉴定的树突特异性蛋白。在这里，我们使用原位杂交与特定的互补DNA探针，以显示树突特异性微管相关蛋白MAP 2（参考文献3-5）的信使RNA存在于发育中的大脑树突。相比之下，微管蛋白的mRNA，存在于轴突和树突的蛋白质4，6，7只位于神经元细胞体。
For nerve cells to develop their highly polarized form, appropriate structural molecules must be targeted to either axons or dendrites. This could be achieved by the synthesis of structural proteins in the cell body and their sorting to either axons or dendrites by specific transport mechanisms. For dendrites, an alternative possibility is that proteins could be synthesized locally in the dendritic cytoplasm. This is an attractive idea because it would allow regulation of the production of structural molecules in response to local demand during dendritic development. The feasibility of dendritic protein synthesis is suggested both by the existence of dendritic polyribosomes1and by the recent demonstration that newly synthesized RNA is transported into the dendrites of neurons differentiating in culture2. However, to date there has been no demonstration of the selective synthesis of an identified dendrite-specific protein in the dendritic cytoplasm. Here, we usein situhybridization with specific complementary DNA probes to show that messenger RNA for the dendrite-specific microtubule-associated protein MAP2 (refs 3–5) is present in dendrites in the developing brain. By contrast the mRNA for tubulin, a protein present in both axons and dendrites4,6,7is located exclusively in neuronal cell bodies.