Breastfeeding linked to the reduction of both rotavirus shedding and IgA levels after Rotarix® immunization in Mexican infants
Breastfeeding linked to the reduction of both rotavirus shedding and IgA levels after Rotarix® immunization in Mexican infants
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DOI：
10.1016/j.vaccine.2016.09.006
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发表时间：
2016-10-17
期刊：
影响因子：
5.5
通讯作者：
Richardson, Vesta.
中科院分区：
文献类型：
--
作者：
Bautista-Marquez, Aurora;Velasquez, Daniel E.;Richardson, Vesta.
我们研究了墨西哥婴儿中疫苗病毒脱落和人轮状病毒疫苗（Rotarix）抗体血清反应的潜在危险因素。在墨西哥城，为进行常规儿童免疫接种（类似于8周龄和15周龄）的前两次访问期间，对婴儿注射了两剂Rotarix。获得婴儿的特征和社会经济指标，包括长期喂养习惯的历史（纯/主要母乳喂养和纯/主要非母乳喂养）。收集了两份血清标本，一份是在第二次轮状病毒疫苗接种期间收集的，另一份是在7周后收集的。在两次轮状病毒疫苗接种后的第4-7天之间收集粪便标本。采用酶免疫测定法（EIA）测定血清中轮状病毒IgA和IgG滴度，采用EIA法测定粪便中轮状病毒的脱落情况，并采用RT-PCR法证实。总的轮状病毒IgA几何平均滴度（GMT）在剂量2后显著增加，从剂量1后的176 （95%CI: 113-273）增加到335 (238-471)；P = 0.020)。纯母乳喂养/主要母乳喂养的婴儿在粪便中传播疫苗病毒的可能性低于配方奶喂养的婴儿（22%对43%，p = 0.016）。母乳喂养的婴儿在剂量1后的轮状病毒IgA滴度低于配方喂养的婴儿[GMT: 145 (84-250) vs. 267 (126-566) p = 0.188]和剂量2 [236 (147-378)vs.578 (367-910), p = 0.007]。接种1剂后出现病毒脱落的婴儿血清IgA GMT明显高于未出现病毒脱落的婴儿[425（188-965）比150 (84-266),p = 0.038]。母乳喂养与减少粪便疫苗脱落和IgA血清反应有关。轮状病毒在肠道中的复制减少以及在第1次剂量后的脱落可能部分解释了血清中较低的IgA反应。Elsevier Ltd.出版。
We examined potential risk factors on vaccine virus shedding and antibody seroresponse to human rota virus vaccine (Rotarix) in Mexican infants. Two doses of Rotarix were administered to infants during the first two visits for their routine childhood immunization (similar to 8 and 15 weeks of age) in Mexico City. Infant's characteristics and socioeconomic indicators were obtained, including history of long-term feeding practices (exclusively/predominantly breastfed and exclusively/predominantly non-breastfed). Two serum specimens were collected, one during the second rotavirus vaccine visit and one 7 weeks later. Stool specimens were collected between days 4-7 after each of the two rotavirus vaccine doses. Rotavirus IgA and IgG titers in serum were determined by enzyme immunoassays (EIA) and rotavirus shedding in stool was assessed by EIA and confirmed by RT-PCR.The overall rotavirus IgA geometric mean titers (GMT) increased significantly post dose 2 from post dose 1 [176 (95%CI: 113-273) to 335 (238-471); p = 0.020). Infants who were exclusively/predominantly breastfed were less likely to shed vaccine virus in stool than those who were formula-fed (22% vs. 43%, p = 0.016). Infants who were breastfed had lower rotavirus IgA titers than those who were formula-fed after dose 1 [GMT: 145 (84-250) vs. 267 (126-566) p = 0.188] and dose 2 [236 (147-378) vs.578 (367-910), p = 0.007]. Infants who shed vaccine virus post dose 1 had significantly higher serum IgA GMT than those who did not shed [425 (188-965) vs. 150 (84-266), p = 0.038]. Breastfeeding was linked with the reduction of both stool vaccine shedding, and IgA seroresponse. The reduced rotavirus replication in the gut and shedding after dose 1 may explain in part the lower IgA response in serum. Published by Elsevier Ltd.