Postsynaptic cell type and synaptic distance do not determine efficiency of monosynaptic rabies virus spread measured at synaptic resolution.
Postsynaptic cell type and synaptic distance do not determine efficiency of monosynaptic rabies virus spread measured at synaptic resolution.
复制标题
突触后细胞类型和突触距离并不能决定狂犬病病毒在突触分辨率下传播的效率。
DOI：
10.7554/elife.89297
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发表时间：
2023-12-14
期刊：
影响因子：
7.7
通讯作者：
Callaway EM
中科院分区：
文献类型：
--
作者：
Patiño M;Lagos WN;Patne NS;Miyazaki PA;Bhamidipati SK;Collman F;Callaway EM
使用糖蛋白缺失的狂犬病病毒的逆行单突触追踪是研究神经回路结构和连接的工具包的重要组成部分。它允许识别中枢和外周神经系统中感兴趣的细胞群的一阶突触前连接，有助于破译引起大脑功能的神经网络的复杂连接模式。尽管它的效用，影响狂犬病跨突触传播的概率的因素还没有得到很好的理解。虽然已经确定用于反式补充G缺失狂犬病的狂犬病糖蛋白的表达水平可以导致每个起始细胞标记的输入数量的巨大变化（收敛指数[CI]），但尚不清楚CI的典型值如何与标记的突触接触或输入神经元的比例相关。我们也不知道不同细胞类型的输入信号，或者离细胞体更近或更远的突触接触信号，是否被标记为不同的概率。在这里，我们使用一种新的狂犬病病毒的结构，允许同时标记的前和突触后的专业化，以量化标记在小鼠初级视觉皮层的突触接触的比例。我们证明，在典型的条件下，约40%的一阶突触前兴奋性突触皮质兴奋性和抑制性神经元的标记。我们发现，使用匹配的跟踪条件下，有相似的比例标记的接触到L4兴奋性锥体，生长抑素（SST）抑制，和血管活性肠肽（VIP）启动细胞类型。此外，我们发现标记的兴奋性接触到突触后位点在不同的亚细胞位置的比例没有差异。
Retrograde monosynaptic tracing using glycoprotein-deleted rabies virus is an important component of the toolkit for investigation of neural circuit structure and connectivity. It allows for the identification of first-order presynaptic connections to cell populations of interest across both the central and peripheral nervous system, helping to decipher the complex connectivity patterns of neural networks that give rise to brain function. Despite its utility, the factors that influence the probability of transsynaptic rabies spread are not well understood. While it is well established that expression levels of rabies glycoprotein used to trans-complement G-deleted rabies can result in large changes in numbers of inputs labeled per starter cell (convergence index [CI]), it is not known how typical values of CI relate to the proportions of synaptic contacts or input neurons labeled. And it is not known whether inputs to different cell types, or synaptic contacts that are more proximal or distal to the cell body, are labeled with different probabilities. Here, we use a new rabies virus construct that allows for the simultaneous labeling of pre- and postsynaptic specializations to quantify the proportion of synaptic contacts labeled in mouse primary visual cortex. We demonstrate that with typical conditions about 40% of first-order presynaptic excitatory synapses to cortical excitatory and inhibitory neurons are labeled. We show that using matched tracing conditions there are similar proportions of labeled contacts onto L4 excitatory pyramidal, somatostatin (Sst) inhibitory, and vasoactive intestinal peptide (Vip) starter cell types. Furthermore, we find no difference in the proportions of labeled excitatory contacts onto postsynaptic sites at different subcellular locations.
影响因子：
25
作者：
Rancz, Ede A.;Franks, Kevin M.;Schwarz, Martin K.;Pichler, Bruno;Schaefer, Andreas T.;Margrie, Troy W.
通讯作者：
Margrie, Troy W.
影响因子：
16.6
作者：
Wickersham IR;Sullivan HA;Seung HS
通讯作者：
Seung HS