Identification of carcinogenic tryptophan pyrolysis products in human bile by high‐performance liquid chromatography
Identification of carcinogenic tryptophan pyrolysis products in human bile by high‐performance liquid chromatography
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高效液相色谱法鉴定人胆汁中致癌色氨酸热解产物
DOI：
10.1002/em.2850150410
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发表时间：
1990
影响因子：
2.8
通讯作者：
O. Wada
中科院分区：
文献类型：
--
作者：
S. Manabe;O. Wada
通过使用高效液相色谱 (HPLC) 方法，已证明致癌色氨酸热解产物 3-氨基-1,4-二甲基-5H-吡啶并[4,3-b]吲哚 (Trp-P-1) 和 3-氨基-1-甲基-5H-吡啶并[4,3-b]吲哚 (Trp-P-2) 存在于人胆汁中。该方法包括酸诱导胆汁成分中致癌物质的释放、用二氯甲烷提取以及随后通过 HPLC 进行定量。在 7 名接受胆管插管和胆外引流的受试者中，每日胆汁中 Trp-P-1 和 Trp-P-2 的平均排泄量分别为 408 fmol/天 (n = 7) 和 864 fmol/天 (n = 7)。此外，在两周的观察期间，证实了一名受试者胆汁和血浆中这些致癌物水平的每日显着变化。这些结果表明这些致癌物的排泄途径之一是通过胆汁。我们的数据还可能表明 Trp-P-1 和 Trp-P-2 源自日常食物。
The carcinogenic tryptophan pyrolysis products 3‐amino‐1,4‐dimethyl‐5H‐pyrido[4,3‐b]indole (Trp‐P‐1) and 3‐amino‐1‐methyl‐5H‐pyrido[4,3‐b]indole (Trp‐P‐2) have been demonstrated to be present in human bile through use of a highperformance liquid chromatography (HPLC) method. The method consists of the acid‐induced release of the carcinogens from bile components and their extraction with methylene chloride and subsequent quantification by HPLC. In seven subjects who had received cath‐eterization of the bile duct and external biliaryd‐rainage, the average amounts of Trp‐P‐1 and Trp‐P‐2 excreted daily in the bile were 408 fmol/ day (n = 7) and 864 fmol/day (n = 7), respectively. In one subject, furthermore, significant daily changes of these carcinogen levels in bile and plasma were confirmed during 2 weeks of observation. These results indicate that one of the excretory pathways of these carcinogens is via bile. Our data also may suggest that Trp‐P‐1 and Trp‐P‐2 are derived from everyday foods.
在熟肉和鱼中检测不到 Trp-P-2。
影响因子：
4.7
作者：
Murray,S;Gooderham,NJ;Barnes,VF;Boobis,AR;Davies,DS
通讯作者：
Davies,DS