microRNA response to Listeria monocytogenes infection in epithelial cells.
microRNA response to Listeria monocytogenes infection in epithelial cells.
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DOI：
10.3390/ijms13011173
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发表时间：
2012
影响因子：
5.6
通讯作者：
Hain T
中科院分区：
文献类型：
--
作者：
Izar B;Mannala GK;Mraheil MA;Chakraborty T;Hain T
microRNA代表一个非常小的非编码RNA家族，其通过拮抗许多靶mRNA来控制若干生理和病理过程，包括宿主免疫应答和癌症。关于细菌感染后microRNA的细胞表达和调节作用的知识有限。我们研究了革兰氏阳性菌感染是否会导致上皮细胞中参与宿主细胞反应的microRNA表达改变。用单核细胞增生李斯特菌EGD-e（突变株（ΔinlAB或Δhly））感染Caco-2细胞或用纯化的溶血素（LLO）孵育。分离总RNA，并使用microRNA微阵列和qRT-PCR将microRNA和靶基因表达与未感染细胞中的表达进行比较。我们鉴定并验证了5种microRNA（miR-146 b、miR-16、let-7a 1、miR-145和miR-155），它们在感染后显著失调。我们发现，特定microRNA的表达模式强烈依赖于病原体定位和细菌效应蛋白的存在。引人注目的是，miR-155被证明在感染期间的炎症反应中具有重要作用，其被野生型细菌、LLO缺陷型细菌以及与纯化的LLO孵育后诱导。它在ΔinlAB感染后下调，表明内化蛋白在细菌致病性和miRNA调控中的新的有效作用。同时，我们观察到所研究的miRNA的靶转录物表达的差异。我们提供了第一个证据，L。单核细胞增多症感染导致在宿主应答中具有重要作用的一组microRNA的失调。不同的microRNA表达取决于LLO和病原体定位。
microRNAs represent a family of very small non-coding RNAs that control several physiologic and pathologic processes, including host immune response and cancer by antagonizing a number of target mRNAs. There is limited knowledge about cell expression and the regulatory role of microRNAs following bacterial infections. We investigated whether infection with a Gram-positive bacterium leads to altered expression of microRNAs involved in the host cell response in epithelial cells. Caco-2 cells were infected with Listeria monocytogenes EGD-e, a mutant strain (ΔinlAB or Δhly) or incubated with purified listeriolysin (LLO). Total RNA was isolated and microRNA and target gene expression was compared to the expression in non-infected cells using microRNA microarrays and qRT-PCR. We identified and validated five microRNAs (miR- 146b, miR-16, let-7a1, miR-145 and miR-155) that were significantly deregulated following listerial infection. We show that expression patterns of particular microRNAs strongly depend on pathogen localization and the presence of bacterial effector proteins. Strikingly, miR-155 which was shown to have an important role in inflammatory responses during infection was induced by wild-type bacteria, by LLO-deficient bacteria and following incubation with purified LLO. It was downregulated following ΔinlAB infection indicating a new potent role for internalins in listerial pathogenicity and miRNA regulation. Concurrently, we observed differences in target transcript expression of the investigated miRNAs. We provide first evidence that L. monocytogenes infection leads to deregulation of a set of microRNAs with important roles in host response. Distinct microRNA expression depends on both LLO and pathogen localization.
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影响因子：
4.4
作者：
Oertli, Mathias;Engler, Daniela B.;Mueller, Anne
通讯作者：
Mueller, Anne
幽门螺杆菌感染过程中 microRNA-155 的诱导及其在炎症反应中的负调节作用
影响因子：
6.4
作者：
Xiao, Bin;Liu, Zhen;Zou, Quan-Ming
通讯作者：
Zou, Quan-Ming
DOI：
10.1007/978-1-61779-037-9_3
发表时间：
2011-01-01
期刊：
ANTIVIRAL RNAI: CONCEPTS, METHODS, AND APPLICATIONS
影响因子：
--
作者：
Plaisance-Bonstaff, Karlie;Renne, Rolf
通讯作者：
Renne, Rolf
影响因子：
4.9
作者：
Pekow, Joel R.;Dougherty, Urszula;Mustafi, Reba;Zhu, Hongyan;Kocherginsky, Masha;Rubin, David T.;Hanauer, Stephen B.;Hart, John;Chang, Eugene B.;Fichera, Alessandro;Joseph, Loren J.;Bissonnette, Marc
通讯作者：
Bissonnette, Marc
影响因子：
11.4
作者：
Schulte, Leon N.;Eulalio, Ana;Vogel, Joerg
通讯作者：
Vogel, Joerg