THE ROLES OF INTERLEUKIN-2 AND INTERFERON-GAMMA IN HUMAN B-CELL ACTIVATION, GROWTH AND DIFFERENTIATION
THE ROLES OF INTERLEUKIN-2 AND INTERFERON-GAMMA IN HUMAN B-CELL ACTIVATION, GROWTH AND DIFFERENTIATION
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DOI：
10.1002/eji.1830160809
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发表时间：
1986-08-01
影响因子：
5.4
通讯作者：
LIPSKY, PE
中科院分区：
文献类型：
--
作者：
JELINEK, DF;SPLAWSKI, JB;LIPSKY, PE
白细胞介素2 （IL 2）和干扰素- γ的作用。（IFN-. γ）对人外周血B细胞活化、生长和分化的影响。在没有添加由丝裂原激活的T细胞产生的T细胞上清液（T sup）的情况下，经金黄色葡萄球菌（SA）刺激的高度纯化的B细胞增殖最小，不产生免疫球蛋白分泌细胞（ISC）。重组IL - 2 （rIL - 2）能够在高纯度sa刺激的B细胞培养中最大限度地促进增殖和ISC的产生。干扰素可以利用。相比之下，不能单独支持任何一种反应。当采用两步培养系统来确定T细胞在初始激活和激活后反应的传播过程中的影响时，发现单独被SA激活的B细胞随后对T sup的反应最大，但对IL 2的反应最小，而对IFN- γ则完全没有反应。然而，tsup， rIL 2或rIFN- γ的存在。发现在SA初始激活过程中，极大地促进了随后激活的B细胞增殖和分化的能力，以响应T支持或IL - 2。这些数据提示了人类B细胞反应的两种不同途径。IFN- γ在T细胞中除IL - 2外的活性。可以支持最初被SA单独激活的B细胞的生长和分化，而IL - 2只有在T细胞淋巴因子（如IL - 2或IFN- γ）存在的情况下才能够最大限度地促进这些反应。结果强调了特异性T细胞因子在决定体液免疫应答结果中的作用。
The roles of interleukin 2 (IL 2) and interferon-.gamma. (IFN-.gamma.) in human peripheral blood B cell activation, growth and differentiation were examined. Highly purified B cells stimulated with Cowan I Staphylococcus aureus (SA) proliferated minimally and generated no immunoglobulin-secreting cells (ISC) without the addition of T cell supernatants (T sup) produced by mitogen-activated T cells. Recombinant IL 2 (rIL 2) alone was able to promote maximum proliferation and generation of ISC in cultures of highly purified SA-stimulated B cells when present from the initiation of the incubation. IFN-.gamma., by contrast, could not support either response alone. When a two-step culture system was employed to determine the effect(s) of T cell influences during both initial activation and in propagating the response following activation, it was found that B cells activated by SA alone subsequently responded maximally to T sup but only minimally to IL 2 and not at all to IFN-.gamma.. However, the presence of T sup, rIL 2, or rIFN-.gamma. during initial activation with SA was found to facilitate greatly the subsequent capacity of the activated B cells to proliferate and differentiate in response to either T sup or IL 2. These data suggest two distinct pathways of human B cell responsiveness. Activities in T sup other than IL 2 of IFN-.gamma. can support the growth and differentiation of B cells initially activated with SA alone, whereas IL 2 is capable of promoting these responses maximally only when B cells have been initially activated by SA in the presence of T cell lymphokines, such as IL 2 or IFN-.gamma.. The results emphasize the role of specific T cell factors in determining the outcome of humoral immune responses.