Gene structure for mouse glutathione reductase, including a putative mitochondrial targeting signal.
Gene structure for mouse glutathione reductase, including a putative mitochondrial targeting signal.
复制标题
小鼠谷胱甘肽还原酶的基因结构，包括假定的线粒体靶向信号。
DOI：
10.1006/bbrc.1997.7153
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Hansen,TN
中科院分区：
文献类型：
--
作者：
Tamura,T;McMicken,HW;Smith,CV;Hansen,TN
谷胱甘肽还原酶（GR）是细胞抗氧化防御功能的重要组成部分。虽然在线粒体和细胞质中发现了GR活性，但哺乳动物GR进入线粒体的分选机制尚未阐明。为了鉴定小鼠GR基因的结构，包括潜在的线粒体靶向信号（MTS）序列，我们筛选了一个小鼠基因组文库，并分离了四个连续的克隆，这些克隆覆盖了该基因的整个编码区。编码区由13个外显子组成。外显子1有两个帧内启动密码子，由富含精氨酸肽段的序列分隔。在表达研究中，将含有小鼠5′外显子1前78 bp的质粒瞬时转染中国仓鼠卵巢细胞，发现线粒体GR活性显著选择性增加。结果表明，这段78bp的序列编码了小鼠GR的潜在MTS。
Glutathione reductase (GR) is an important component of cellular antioxidant defense functions. Although GR activities are found in mitochondria and cytoplasm, the sorting mechanisms of mammalian GR into mitochondria have not been elucidated. To identify the mouse GR gene structure, including a sequence for a potential mitochondrial targeting signal (MTS), we screened a mouse genomic library and isolated four contiguous clones that covered the entire coding region of the gene. The coding region is composed of 13 exons. Exon 1 has two in-frame start codons separated by a sequence for an arginine-rich peptide segment. Expression studies, in which Chinese hamster ovary cells were transiently transfected with a plasmid containing the first 78 bp of the mouse exon 1 attached 5′ to the human GR cDNA, showed marked and selective increases in mitochondrial GR activities. The data indicate that this 78 bp sequence encodes a potential MTS for GR in mice.