The PDZ domain of OutC and the N-terminal region of OutD determine the secretion specificity of the type II out pathway of Erwinia chrysanthemi
The PDZ domain of OutC and the N-terminal region of OutD determine the secretion specificity of the type II out pathway of Erwinia chrysanthemi
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DOI：
10.1006/jmbi.2001.4594
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发表时间：
2001-04-27
影响因子：
5.6
通讯作者：
Shevchik, VE
中科院分区：
文献类型：
--
作者：
Bouley, J;Condemine, G;Shevchik, VE
植物病原体菊欧文氏菌和胡萝卜软腐欧文氏菌通过 II 型途径（Out 系统）分泌多种外蛋白。欧文氏菌的分泌具有物种特异性：一种物种的外蛋白不能被另一种物种分泌。我们分析了 Out 系统的两个组件（双位内膜蛋白 OutC 和促胰液素 OutD）在分泌蛋白特异性识别中的作用。我们证明 OutC 的 PDZ 结构域决定了其对某些外蛋白的分泌特异性。促胰液素是 Out 系统特异性的主要决定因素：胡萝卜软腐菌的 OutD 改变了菊花 E. chrysanthemi 的分泌特异性，使其能够分泌异源外蛋白。嵌合OutD的构建表明N端区域是促胰液素的特异性结构域。因此，OutC 的 PDZ 结构域和 OutD 的 N 末端区域都是分泌蛋白特异性识别所必需的。对几种外蛋白分泌的系统分析表明，Out 机制分泌的不同外蛋白对其在 OutC 和 OutD 上的假定靶向信号有不同的要求。这强烈表明不同的外蛋白具有不同数量的靶向信号，这些信号被 OutC 和 OutD 的不同区域识别。 (C) 2001 年学术出版社。
The plant pathogens Erwinia chrysanthemi and Erwinia carotovora secrete multiple exoproteins by a type II pathway, the Out system. Secretion in Erwinia is species-specific: exoproteins of one species cannot be secreted by the other. We analysed the role of two components of the Out system, the bitopic inner membrane protein OutC and the secretin OutD, in the specific recognition of secreted proteins. We demonstrated that the PDZ domain of OutC determines its secretion specificity towards certain exoproteins. The secretin is the major determinant of specificity of the Out system: OutD of E. carotovora changes the secretion specificity of E. chrysanthemi and enables it to secrete heterologous exoproteins. Construction of chimeric OutD showed that the N-terminal region is the specificity domain of the secretin. Thus, both the PDZ domain of OutC and the N-terminal region of OutD are required for specific recognition of secreted proteins. Systematic analysis of the secretion of several exoproteins demonstrated that different exoproteins secreted by the Out machinery have different requirement for their presumed targeting signals on OutC and OutD. This strongly indicates that diverse exoproteins possess a variable number of targeting signals which are recognised by different regions of OutC and OutD. (C) 2001 Academic Press.