Jasmonate-Responsive ERF Transcription Factors Regulate Steroidal Glycoalkaloid Biosynthesis in Tomato
Jasmonate-Responsive ERF Transcription Factors Regulate Steroidal Glycoalkaloid Biosynthesis in Tomato
复制标题
DOI：
10.1093/pcp/pcw067
复制
发表时间：
2016-05-01
影响因子：
4.9
通讯作者：
Shoji, Tsubasa
中科院分区：
文献类型：
--
作者：
Thagun, Chonprakun;Imanishi, Shunsuke;Shoji, Tsubasa
甾体糖生物碱（SGAs）是在茄科植物中产生的胆固醇衍生的特殊代谢物。本文报道了乙烯响应因子（ERF）家族（JREs）的一组茉莉素响应转录因子与Cathranthus roseus和烟草中的生物碱调节因子具有密切的同源性，并调节番茄中SGAs的产生。在转基因番茄中，JRE基因的过表达和显性抑制导致SGA积累和涉及SGA生物合成多步骤途径的代谢酶基因表达发生剧烈变化，包括甲羟戊酸上游途径。反激活和dna -蛋白结合实验表明，JRE4通过结合启动子中的GCC盒状元件激活SGA生物合成基因的转录。这些JRE结合元件在JRE基因调控基因的近端启动子区域出现的频率明显更高，支持JREs介导一系列参与SGA生物合成的代谢基因的转录协调的结论。
Steroidal glycoalkaloids (SGAs) are cholesterol-derived specialized metabolites produced in species of the Solanaceae. Here, we report that a group of jasmonate-responsive transcription factors of the ETHYLENE RESPONSE FACTOR (ERF) family (JREs) are close homologs of alkaloid regulators in Cathranthus roseus and tobacco, and regulate production of SGAs in tomato. In transgenic tomato, overexpression and dominant suppression of JRE genes caused drastic changes in SGA accumulation and in the expression of genes for metabolic enzymes involved in the multistep pathway leading to SGA biosynthesis, including the upstream mevalonate pathway. Transactivation and DNA-protein binding assays demonstrate that JRE4 activates the transcription of SGA biosynthetic genes by binding to GCC box-like elements in their promoters. These JRE-binding elements occur at significantly higher frequencies in proximal promoter regions of the genes regulated by JRE genes, supporting the conclusion that JREs mediate transcriptional co-ordination of a series of metabolic genes involved in SGA biosynthesis.