Comparison of porcine parvovirus to other parvoviruses by restriction site mapping and hybridization analysis of Southern Blots.
Comparison of porcine parvovirus to other parvoviruses by restriction site mapping and hybridization analysis of Southern Blots.
复制标题
通过限制性位点作图和 Southern 印迹杂交分析比较猪细小病毒与其他细小病毒。
DOI：
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发表时间：
1987
影响因子：
3.8
通讯作者：
W. Mengeling
中科院分区：
文献类型：
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作者：
J. Ridpath;P. Paul;W. Mengeling
通过限制性内切酶切点分析和杂交分析，研究了猪细小病毒(PPV)与其他几种自治细小病毒之间的基因组关系。用8种限制性内切酶中的每一种分别消化病毒DNA的双链复制形式，从而绘制PPV基因组的限制位点图。随后将这些图谱与先前报道的PPV、犬细小病毒(CPV)、猫泛白细胞减少症病毒(FPV)、小鼠微小病毒(MVM)、H-1病毒(H-1)和牛细小病毒(BPV)的图谱进行比较，发现尽管CPV、FPV、MVM和H-1的图谱有许多共同的特征，但PPV和BPV的图谱有很大不同。为了进行杂交分析，将PPV、CPV和BPV基因组缺口翻译制备的放射性探针分别在低和高两种条件下进行同源杂交和与其他两种病毒以及与FPV的异源杂交。这些测试的结果表明，PPV、CPV和FPV的基因组之间是同源性的，但BPV的基因组与任何其他被测试的病毒之间几乎没有同源性。用PPV的限制性片段和CPV探针进行的进一步测试表明，异源杂交主要局限于基因组3‘端1.85-2.7kb的片段。根据先前确定的几种啮齿动物细小病毒的转录图谱，该区间可能包括非结构蛋白和结构蛋白的部分编码序列，可能是PPV与CPV和FPV之间复制和抗原相似性的遗传基础。
The genomic relationship between porcine parvovirus (PPV) and several other autonomous parvoviruses was examined by restriction site and hybridization analysis. Restriction site maps of the PPV genome were prepared by digesting the double-stranded replicative form of the viral DNA with each of eight restriction enzymes. Subsequent comparison of such maps with those previously reported for PPV, canine parvovirus (CPV), feline panleukopenia virus (FPV), minute virus of mice (MVM), H-1 virus (H-1) and bovine parvovirus (BPV) revealed that while the maps of CPV, FPV, MVM and H-1 had a number of features in common, those of PPV and BPV were substantially different. For hybridization analysis radioactive probes prepared by nick translation of PPV, CPV and BPV genomes were tested under conditions of both low and high stringency for homologous hybridization and for heterologous hybridization with each of the other two viruses and with FPV. The results of these tests indicated homology among the genomes of PPV, CPV and FPV, but little or no homology between the genome of BPV and those of any of the other viruses tested. Additional tests with restriction fragments of PPV and a CPV probe indicated that heterologous hybridization was confined primarily to a segment of the genome between 1.85 and 2.7 kb from the 3' end. Based on transcriptional maps previously determined for several of the rodent parvoviruses, this interval is likely to include part of the coding sequences for both non-structural and structural proteins and may be the genetic basis for the replicative as well as the antigenic similarities between PPV and both CPV and FPV.