Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012
Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012
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DOI：
10.1016/j.virol.2016.04.026
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发表时间：
2016-08-01
期刊：
影响因子：
3.7
通讯作者：
Vijaykrishna, Dhanasekaran
中科院分区：
文献类型：
--
作者：
Donato, Celeste;Le Thi Hoi;Vijaykrishna, Dhanasekaran
背景资料：2011-2012年期间，肠道病毒71亚基因组C4在越南引起了最大规模的手足口病（HFMD）爆发，导致超过20万人住院，207人死亡。方法：从越南各地的手足口病住院患者中收集了1917份具有足够体积的RT-PCR分析样本，其中637份为EV 71阳性。对87例患者进行了VP 1基因测序，对9例患者进行了全基因组测序。对检测到的B5、C4和C5菌株进行最大似然系统发育分析。结果：序列分析显示，与2012年疫情相关的优势亚基因组为C4，B5和C5菌株占这些病例的一小部分。该地区的许多国家，包括马来西亚，台湾和中国大陆对越南的毒株多样性有很大影响，了解EV 71在整个东南亚的传播对于预防性公共卫生措施和疫苗开发工作至关重要。(C)2016年6月，作者。爱思唯尔公司出版
Background: Enterovirus 71 subgenogroup C4 caused the largest outbreak of Hand, Foot and Mouth Disease (HFMD) in Vietnam during 2011-2012, resulting in over 200,000 hospitalisations and 207 fatalities.Methods: A total of 1917 samples with adequate volume for RT-PCR analysis were collected from patients hospitalised with HFMD throughout Vietnam and 637 were positive for EV71. VP1 gene (n=87) and complete genome (n=9) sequencing was performed. Maximum-likelihood phylogenetic analysis was performed to characterise the B5, C4 and C5 strains detected.Results: Sequence analyses revealed that the dominant subgenogroup associated with the 2012 outbreak was C4, with B5 and C5 strains representing a small proportion of these cases.Conclusions: Numerous countries in the region including Malaysia, Taiwan and China have a large influence on strain diversity in Vietnam and understanding the transmission of EV71 throughout Southeast Asia is vital to inform preventative public health measures and vaccine development efforts. (C) 2016 The Authors. Published by Elsevier Inc.