Increased expression of caveolin-1 and-2 in the hearts of Lewis rats with experimental autoimmune myocarditis
Increased expression of caveolin-1 and-2 in the hearts of Lewis rats with experimental autoimmune myocarditis
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DOI：
10.1080/08916930600929321
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发表时间：
2006-09-01
期刊：
影响因子：
3.5
通讯作者：
Shin, Taekyun
中科院分区：
文献类型：
--
作者：
Ahn, Meejung;Kim, Heechul;Shin, Taekyun
通过研究小窝蛋白-1、-2和-3在实验性自身免疫性心肌炎(EAM)大鼠心脏中的表达，探讨小窝蛋白在EAM发病机制中的作用。Western印迹分析显示，免疫后第14天，EAM组大鼠心脏小窝蛋白-1和-2的表达水平显著高于正常对照组(P<；0.05，正常对照组与EAM组相比)。免疫组织化学显示，小窝蛋白-1主要表达于ED1阳性的巨噬细胞以及EAM病变的部分心肌细胞和血管中。Caveolin-2在正常心脏血管内皮细胞中有组成性表达，EAM大鼠血管内皮细胞中Caveolin-2的表达高于正常对照组。Caveolin-3在EAM病变的心肌细胞膜上呈结构性表达，而在血管内皮细胞和炎性细胞中不表达。我们的结果表明，小窝蛋白-1和小窝蛋白-2在EAM大鼠皮损中的表达增加，并且小窝蛋白-1的表达增加刺激了EAM大鼠受影响的细胞，如巨噬细胞和一些心肌细胞的第二信号。
The expression of caveolin-1, -2 and -3 was studied in the hearts of rats with experimental autoimmune myocarditis (EAM), to elucidate the involvement of caveolins in the pathogenesis of EAM. Western blot analysis showed that levels of caveolin-1 and -2 were significantly increased in the hearts of rats with EAM on day 14 post-immunization ( pi), as compared to the hearts of normal controls (p < 0.05, normal controls vs. EAM). Caveolin-3 is already at a high level in control animals, so it does not increase further.Immunohistochemistry showed that caveolin-1 was expressed mainly in ED1-positive macrophages and in some cardiomyocytes and vessels in the EAM lesions. Caveolin-2 was expressed constitutively in the vascular endothelial cells of normal hearts, and its expression was enhanced in EAM rats, as compared with the normal control group. Caveolin-3 was expressed constitutively in the plasma membranes of cardiomyocytes, but not in the vascular endothelial cells and inflammatory cells in the EAM lesions. Our results suggest that the expression of caveolin-1 and -2 is increased in EAM lesions and that the increased expression of caveolin-1 stimulates second signals in affected cells, such as macrophages and some cardiomyocytes, in EAM rats.