Myocardial recruitment during ANF mRNA increase with volume overload in the rat.
Myocardial recruitment during ANF mRNA increase with volume overload in the rat.
复制标题
ANF mRNA 期间的心肌募集随着大鼠容量超负荷而增加。
DOI：
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发表时间：
1986
影响因子：
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通讯作者：
Florent Soubrier
中科院分区：
文献类型：
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作者：
A. Lattion;Michel Jb;E. Arnauld;P. Corvol;Florent Soubrier
合成的寡核苷酸探针互补信使核糖核酸（mRNA）编码的C-末端部分的心钠素（ANF）已被用来研究的表达的心钠素基因在大鼠心肌。四个实验模型进行了研究：双肾切除术（48小时），结扎的两个输尿管（48小时），脱氧皮质酮醋酸盐（3周）和下腔静脉瘘（2周）。通过凝胶印迹杂交分析心房RNA检测到一条带，其长度与编码ANF的mRNA的长度相对应。在心室RNA中也检测到这种mRNA，但其丰度为1/50。4个实验组心钠素mRNA表达均较对照组显著增加。所有大鼠左、右心房心钠素mRNA含量无显著性差异。每种实验条件都伴随着左心室中ANF基因表达的高度显著增加，其中所有心室组织都可以被募集，并且从左心室的基部到心尖具有负梯度。原位杂交证实了这些数据。因此，所有的心房和心室肌都可以表达ANF基因。心腔被动牵张时募集增加。
A synthetic oligonucleotide probe complementary to messenger ribonucleic acid (mRNA) encoding for the C-terminal portion of atrial natriuretic factor (ANF) has been used to study the expression of the ANF gene in rat myocardium. Four experimental models were studied: binephrectomy (for 48 h); ligature of both ureters (for 48 h); deoxycorticosterone acetate-salt (for 3 wk); and aortocaval fistula (for 2 wk). Analysis of atrial RNA by gel-blot hybridization detected a single band, corresponding in length to that of mRNA coding for ANF. Such an mRNA was also detected in ventricular RNA but was 1/50th as abundant. In the four experimental groups ANF mRNA was increased significantly as compared with controls. In all rats there was no significant difference in the ANF mRNA content between the left and the right atrium. Each experimental condition was accompanied by a highly significant increase in ANF gene expression in the left ventricle, where all of the ventricular tissue could be recruited and with a negative gradient from the base to the apex of the left ventricle. These data were confirmed by in situ hybridization. Thus all of the atrial and ventricular myocardium can express the ANF gene. Recruitment increases in response to passive stretch of the cardiac chambers.