Characterization of the maize xylem sap proteome
Characterization of the maize xylem sap proteome
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DOI：
10.1021/pr050471q
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发表时间：
2006-04-01
影响因子：
4.4
通讯作者：
Schachtman, DP
中科院分区：
文献类型：
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作者：
Alvarez, S;Goodger, JQD;Schachtman, DP
植物中的木质部主要被描述为水和矿物质的管道，但新出现的证据也表明木质部含有蛋白质。为了研究木质部汁液中的蛋白质，我们对玉米木质部汁液蛋白质组的组成和特性进行了研究。玉米木质部汁液蛋白质组的组成揭示了与木质部分化的不同阶段相关的蛋白质，包括细胞壁代谢、次生细胞壁合成和程序性细胞死亡。发现许多蛋白质以多种亚型存在，并且这些亚型中的一些是糖基化的。木质部汁液中还存在与防御机制有关的蛋白质，生物测定表明汁液蛋白具有抗真菌活性。
The xylem in plants has mainly been described as a conduit for water and minerals, but emerging evidence also indicates that the xylem contains protein. To study the proteins in xylem sap, we characterized the identity and composition of the maize xylem sap proteome. The composition of the xylem sap proteome in maize revealed proteins related to different phases of xylem differentiation including cell wall metabolism, secondary cell wall synthesis, and programmed cell death. Many proteins were found to be present as multiple isoforms and some of these isoforms are glycosylated. Proteins involved in defense mechanisms were also present in xylem sap and the sap proteins were shown to have antifungal activity in bioassays.