Role of HNF4alpha in the superinduction of the IL-1beta-activated iNOS gene by oxidative stress.
Role of HNF4alpha in the superinduction of the IL-1beta-activated iNOS gene by oxidative stress.
复制标题
HNF4α 在氧化应激诱导 IL-1β 激活的 iNOS 基因中的作用。
DOI：
10.1042/bj20060005
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Gonzalez,FrankJ
中科院分区：
文献类型：
--
作者：
Gonzalez,FrankJ
IL-1β (interleukin-1β) treatment of hepatocytes results in an NF-κB (nuclear factor-κB)-mediated activation of the iNOS (induced nitric oxide synthase) gene, and this increase in gene expression is further augmented by oxidative stress. Oxidative stress alone has no influence on the iNOS promoter, therefore indicating that the promoter needs to be primed by NF-κB. In this issue of theBiochemical Journal, Guo et al. extend their earlier work, showing that HNF4α (hepatocyte nuclear factor 4α) mediates the superinduction of iNOS observed by co-treating cells with IL-1β plus H2O2. A specific phosphorylation by p38 kinase at Ser-158 of HNF4α results in increased binding of HNF4α to the iNOS promoter, leading to enhanced transcription. The study by Guo et al. is the first to show definitively that HNF4α can be modulated to differentially activate specific genes. However, issues remain to determine the functional significancein vivoof the elevated iNOS activity, and the mechanism that governs the specificity of HNF4α towards the iNOS promoter element as compared with many other HNF4α target genes in the hepatocyte.
