TaSCL14, a Novel Wheat (Triticum aestivum L.) GRAS Gene, Regulates Plant Growth, Photosynthesis, Tolerance to Photooxidative Stress, and Senescence
TaSCL14, a Novel Wheat (Triticum aestivum L.) GRAS Gene, Regulates Plant Growth, Photosynthesis, Tolerance to Photooxidative Stress, and Senescence
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TaSCL14 是一种新型小麦 (Triticum aestivum L.) GRAS 基因，调节植物生长、光合作用、光氧化应激耐受性和衰老
DOI：
10.1016/j.jgg.2014.11.002
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发表时间：
2015-01-20
影响因子：
5.9
通讯作者：
Li, Zhensheng
中科院分区：
文献类型：
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作者：
Chen, Kunmei;Li, Hongwei;Li, Zhensheng
光合速率、对光氧化胁迫的耐受性和衰老都是影响植物发育并因此影响农业生产力的重要生理因素。GRAS蛋白在植物生长发育以及植物对生物和非生物胁迫的响应中起着重要作用。迄今为止，在小麦（Triticum aestivum L.）已经被定性。先前的转录组分析表明，GRAS基因（TaSCL 14）的表达是由强光胁迫诱导的小偃54（XY54），一个普通小麦品种具有较强的耐强光胁迫。本研究从XY54中克隆了TaSCL 14基因，并将其定位在染色体4A上。TaSCL 14在小麦的不同器官中均有表达，在茎和根中表达量较高。我们的结果证实了TaSCL 14的表达确实对强光胁迫有反应。利用大麦条纹花叶病毒（Barley stripe mosaic virus，BSMV）诱导小麦TaSCL 14基因沉默（VIGS），研究TaSCL 14基因的功能。TaSCL 14的沉默导致植物生长受到抑制，光合能力降低，对光氧化胁迫的耐受性降低。此外，TaSCL 14基因的沉默促进了黑暗诱导的叶片衰老。这些结果表明，TaSCL 14可能作为一个多功能的调节剂参与植物生长，光合作用，耐光氧化胁迫，衰老。
Rates of photosynthesis, tolerance to photooxidative stress, and senescence are all important physiological factors that affect plant development and thus agricultural productivity. GRAS proteins play essential roles in plant growth and development as well as in plant responses to biotic and abiotic stresses. So far few GRAS genes in wheat (Triticum aestivum L.) have been characterized. A previous transcriptome analysis indicated that the expression of a GRAS gene (TaSCL14) was induced by high-light stress in Xiaoyan 54 (XY54), a common wheat cultivar with strong tolerance to high-light stress. In this study, TaSCL14 gene was isolated from XY54 and mapped on chromosome 4A. TaSCL14 was expressed in various wheat organs, with high levels in stems and roots. Our results confirmed that TaSCL14 expression was indeed responsive to high-light stress. Barley stripe mosaic virus (BSMV)-based virus-induced gene silencing (VIGS) of TaSCL14 in wheat was performed to help characterize its potential functions. Silencing of TaSCL14 resulted in inhibited plant growth, decreased photosynthetic capacity, and reduced tolerance to photooxidative stress. In addition, silencing of TaSCL14 in wheat promoted leaf senescence induced by darkness. These results suggest that TaSCL14 may act as a multifunctional regulator involved in plant growth, photosynthesis, tolerance to photooxidative stress, and senescence.