Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture
Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture
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DOI：
10.1016/j.biomaterials.2007.08.038
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发表时间：
2007-12-01
期刊：
影响因子：
14
通讯作者：
Kane, Ravi S.
中科院分区：
文献类型：
--
作者：
Ashton, Randolph S.;Banerjee, Akhilesh;Kane, Ravi S.
我们描述了一种具有可调节降解速率的藻酸盐水凝胶的方法，该方法可以用作干细胞的支架。藻酸盐水凝胶已被广泛测试为细胞培养的三维构建体，用于植入的细胞载体以及在组织再生应用中。但是，藻酸盐水凝胶植入物可能需要几个月的时间才能从植入部位消失，因为哺乳动物不产生内源性藻酶。通过掺入含有藻酸盐裂解酶的聚（乳酸 - 糖糖苷）（PLGA）微球中，我们证明了藻酸盐水凝胶可以以可控且可调的方式降解藻酸水凝胶。我们证明，在该可降解的藻酸盐水凝胶系统中，可以在体外培养和扩展神经祖细胞（NPC）。此外，我们观察到在降解藻酸盐水凝胶中培养的NPC的膨胀率显着提高，而不是标准培养的NPC，即非降解，藻酸盐水凝胶。封装干细胞的可降解藻酸盐水凝胶可以广泛应用于开发新的组织再生疗法。 （c）2007 Elsevier Ltd.保留所有权利。
We describe a method for creating alginate hydrogels with adjustable degradation rates that can be used as scaffolds for stem cells. Alginate hydrogels have been widely tested as three-dimensional constructs for cell culture, cell carriers for implantation, and in tissue regeneration applications; however, alginate hydrogel implants can take months to disappear from implantation sites because mammals do not produce endogenous alginases. By incorporating poly(lactide-co-glycolide) (PLGA) microspheres loaded with alginate lyase into alginate hydrogels, we demonstrate that alginate hydrogels can be enzymatically degraded in a controlled and tunable fashion. We demonstrate that neural progenitor cells (NPCs) can be cultured and expanded in vitro in this degradable alginate hydrogel system. Moreover, we observe a significant increase in the expansion rate of NPCs cultured in degrading alginate hydrogels versus NPCs cultured in standard, i.e. non-degrading, alginate hydrogels. Degradable alginate hydrogels encapsulating stem cells may be widely applied to develop novel therapies for tissue regeneration. (C) 2007 Elsevier Ltd. All rights reserved.