Effects of ageing and smoking on SP-A and SP-D levels in bronchoalveolar lavage fluid
Effects of ageing and smoking on SP-A and SP-D levels in bronchoalveolar lavage fluid
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DOI：
10.1183/09031936.04.00064004
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发表时间：
2004-12-01
影响因子：
24.3
通讯作者：
Nishimura, M
中科院分区：
文献类型：
--
作者：
Betsuyaku, T;Kuroki, Y;Nishimura, M
表面活性剂蛋白(SP)-A和SP- d是在远端肺上皮中合成的胶原样糖蛋白。这项研究考察了衰老和长期吸烟对肺部SP-A和SP-D的影响。还调查了与肺气肿发展的可能联系。序贯灌洗对青壮年、中年或老年不吸烟者和有不同吸烟史的无症状吸烟者进行。通过高分辨率计算机断层扫描进一步对中老年吸烟者进行肺气肿分类。ELISA法测定支气管灌洗液（BL）和支气管肺泡灌洗液（BAL）中SP-A和SP-D的水平。非吸烟者BL液中SP-A随年龄显著降低，BAL液中SP-A无明显下降。与年轻受试者相比，患有肺气肿的中年或老年吸烟者的BL和BAL液中的SPA含量较低，与没有肺气肿的中年或老年吸烟者相比，BL液中的SPA含量也较低。SP-D不随年龄变化，然而，与年龄相仿的非吸烟者相比，中年或老年吸烟者的SP-D水平有所下降。综上所述，表面活性剂蛋白a可能随着年龄的增长或长期吸烟和肺气肿的发展而降低，而表面活性剂蛋白d则可能因长期吸烟而降低。
Surfactant protein (SP)-A and SP-D are collagen-like glycoproteins that are synthesised in the distal pulmonary epithelium. This study examined the effects of ageing and long-term smoking on SP-A and SP-D in the lungs. The possible links to the development of pulmonary emphysema were also investigated.Sequential lavage was performed in young and middle-aged or elderly nonsmokers and asymptomatic current smokers with various smoking histories. Middle-aged or elderly smokers were further categorised according to the presence of emphysema by high-resolution computed tomography. Levels of SP-A and SP-D in bronchial lavage (BL) fluid and in bronchoalveolar lavage (BAL) fluid were quantified by ELISA.Significant decreases in SP-A were seen with age in nonsmokers in BL fluid, but not in BAL fluid. Middle-aged or elderly smokers with emphysema had lower levels of SPA in both BL and BAL fluids when compared with young subjects, and in BL fluid when compared with middle-aged or elderly smokers without emphysema. SP-D did not change with age alone, however, it was decreased in middle-aged or elderly smokers when compared with similarly aged nonsmokers.In conclusion, surfactant protein-A may decrease with age alone or due to the cumulative effects of long-term smoking and development of emphysema, while surfactant protein-D decreases due to long-term smoking.