The cardiac neural crest gene MafB ectopically directs CXCR4 expression in the trunk neural crest
The cardiac neural crest gene MafB ectopically directs CXCR4 expression in the trunk neural crest
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DOI：
10.1016/j.ydbio.2022.12.006
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发表时间：
2022-12-26
影响因子：
2.7
通讯作者：
Inoue，Kunio
中科院分区：
文献类型：
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作者：
Tani-Matsuhana，Saori;Kawata，Yuga;Inoue，Kunio
心脏神经嵴是从尾侧后脑产生的细胞亚群。分层的心脏神经嵴细胞使用CXCR/SDF 1趋化因子信号系统迁移到心脏。这些细胞有助于心血管系统的形成，包括流出道的分隔，这是这些细胞所特有的。在这里，我们研究了心脏神经嵴基因MafB的异位表达对躯干神经嵴细胞的影响。首先，我们发现MafB有可能激活其自身的顺式调节元件在肠道和躯干神经嵴细胞，但不是在颅神经嵴细胞。两个心脏神经嵴基因Ets 1和Sox 8的强制表达，加上或without MafB，诱导异位Sox 10 E2增强子活性的躯干区域。最后，我们发现MafB，Ets 1和Sox 8的表达可以诱导CXCR 4在躯干神经嵴细胞中的异位表达，导致获得对SDF 1信号的反应性。这些结果表明，MafB，Ets 1和Sox 8是产生心脏神经嵴的身份，特别是细胞迁移特性的关键组分。
The cardiac neural crest is a subpopulation of cells arising from the caudal hindbrain. The delaminated cardiac neural crest cells migrate to the heart using the CXCR/SDF1 chemokine signaling system. These cells contribute to the formation of the cardiovascular system, including the septation of the outflow tract, which is unique to these cells. Here, we investigated the effect of ectopic expression of the cardiac neural crest geneMafBon trunk neural crest cells. First, we found that MafB has the potential to activate its own cis-regulatory element in enteric and trunk neural crest cells but not in cranial neural crest cells. Forced expression of two cardiac neural crest genes,Ets1andSox8, together with or withoutMafB, induced ectopic Sox10E2 enhancer activity in the trunk region. Finally, we uncovered that the expression of MafB, Ets1 and Sox8 can induce ectopic CXCR4 expression in the trunk neural crest cells, resulting in acquisition of responsiveness to the SDF1 signal. These results demonstrate that MafB, Ets1 and Sox8 are critical components for generation of the identity of the cardiac neural crest, especially the cell migration property.