THE DETECTION AND ESTIMATION OF BRANCHING IN DEXTRAN BY PROTON MAGNETIC RESONANCE SPECTROSCOPY
THE DETECTION AND ESTIMATION OF BRANCHING IN DEXTRAN BY PROTON MAGNETIC RESONANCE SPECTROSCOPY
复制标题
质子磁共振波谱法检测和估计右旋糖酐中的支化
DOI：
10.1139/v63-045
复制
发表时间：
1963
影响因子：
1.1
通讯作者：
L. H. Cragg
中科院分区：
文献类型：
--
作者：
W. Pasika;L. H. Cragg
支化葡聚糖的质子磁共振谱中有一个峰，这是线性葡聚糖的谱中没有的，它归属于非1，6-键上的C1质子，在大多数支化的葡聚糖中，这些键形成支化点。分支程度的定量衡量--1，6-键与非1，6-键的比率--是通过取与这两类键相关的峰面积的比率来获得的。对于已知非1，6-键位于分支点的多糖，n.m.r.因此，光谱学提供了一种比目前使用的方法更方便的检测和估计分支的方法。
The proton magnetic resonance spectrum of branched dextran contains a peak, not found in the spectrum of linear dextran, which is assigned to C1 protons at non-1,6-linkages—linkages which in most branched dextrans form the branch points. A quantitative measure of the extent of branching—the ratio of 1,6-linkages to non-1,6-linkages—is obtained by taking the ratio of the areas of the peaks associated with these two types of linkages. The value so obtained agrees well with that obtained by periodate analysis.For polysaccharides in which the non-1,6-linkages are known to be at branch points, n.m.r. spectroscopy thus affords a means of detecting and estimating branching which is more convenient than methods currently in use.