A multiple stress-responsive gene ERD15 from Solanum pennellii confers stress tolerance in tobacco.
A multiple stress-responsive gene ERD15 from Solanum pennellii confers stress tolerance in tobacco.
复制标题
DOI：
10.1093/pcp/pcr057
复制
发表时间：
2011-06
影响因子：
4.9
通讯作者：
Khurram Ziaf;R. Loukehaich;Pengjuan Gong;Hui Liu;Qinqin Han;Taotao Wang;Hanxia Li;Z. Ye
中科院分区：
文献类型：
--
作者：
Khurram Ziaf;R. Loukehaich;Pengjuan Gong;Hui Liu;Qinqin Han;Taotao Wang;Hanxia Li;Z. Ye
野生物种通常比栽培物种对环境压力因素表现出更强的耐受性。从耐旱和耐盐的野生番茄 Solanum pennellii (SpERD15) 中克隆了一个早期脱水反应基因。 SpERD15转录本在不同器官中的积累存在差异，并且受脱水、盐度、寒冷和植物生长调节剂处理的显着诱导。 SpERD15 编码的蛋白质主要位于细胞核中。有趣的是，我们发现大多数转基因烟草植物与过度表达品系一起受到共同抑制。与共抑制株系相比，过表达植物表现出胁迫耐受性，伴随着更多可溶性糖和脯氨酸的积累，并且限制了脂质过氧化，共抑制株系比野生型更敏感。不同转基因品系中这些相容性溶质的差异含量与参与一些重要渗透剂（P5CS和蔗糖合酶）产生的基因表达的变化有关。多种应激因素下脂质过氧化的减少与应激反应基因（ADH 和 GAPDH）表达的增加相一致。 SpERD15 的过表达通过维持 PSII 醌受体处于部分氧化形式，提高了转基因烟草植物中 PSII (F(v)/F(m)) 的效率。结果表明，SpERD15 通过保护细胞膜增强 PSII 的效率，从而增强应激耐受性，这是由相容性溶质的积累和有限的脂质过氧化所赋予的。
Wild species often show more tolerance to environmental stress factors than their cultivated counterparts. An early responsive-to-dehydration gene was cloned from a drought- and salt-tolerant wild tomato Solanum pennellii (SpERD15). SpERD15 transcript accumulated differentially in different organs, and was remarkably induced by dehydration, salinity, cold and treatment with plant growth regulators. The protein encoded by SpERD15 was predominantly localized in the nucleus. Interestingly, we found that the majority of the transgenic tobacco plants were co-suppressed along with the overexpressing line. Overexpressing plants manifested stress tolerance accompanied by the accumulation of more soluble sugars and proline, and limited lipid peroxidation compared with co-suppression lines, which were more sensitive than the wild type. The differential contents of these compatible solutes in different transgenic lines were related to the changes in the expression of the genes involved in the production of some important osmolytes (P5CS and Sucrose synthase). Reduced lipid peroxidation over a broad range of stress factors was in agreement with increased expression of stress-responsive genes (ADH and GAPDH). Overexpression of SpERD15 increased the efficiency of PSII (F(v)/F(m)) in transgenic tobacco plants by maintaining PSII quinone acceptors in a partially oxidized form. The results show that SpERD15 augments stress tolerance by enhancing the efficiency of PSII through the protection of cellular membranes, as conferred by the accumulation of compatible solutes and limited lipid peroxidation.