Cytokine secretion patterns of NK cells and macrophages in early human pregnancy decidua and blood:: Implications for suppressor macrophages in decidua
Cytokine secretion patterns of NK cells and macrophages in early human pregnancy decidua and blood:: Implications for suppressor macrophages in decidua
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DOI：
10.1046/j.8755-8920.2003.00112.x
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发表时间：
2003-12-01
影响因子：
3.6
通讯作者：
Ekerfelt, C
中科院分区：
文献类型：
--
作者：
Lidström, C;Matthiesen, L;Ekerfelt, C
问题：局部免疫调节已被证明对维持成功妊娠相当重要。我们曾报道过人早孕蜕膜分泌干扰素-γ、白介素4和白介素10。本研究的目的是研究蜕膜细胞因子分泌的细胞来源，并将其与外周血的分泌模式进行比较。研究方法：从20名妊娠早期接受手术流产的妇女身上采集蜕膜组织和外周血。结果：蜕膜及外周血单核/巨噬细胞和NK细胞可自发分泌干扰素-γ、IL-4和IL-10。蜕膜巨噬细胞分泌IL-10的细胞数显著高于蜕膜非单核细胞和单核/巨噬细胞。结论：蜕膜巨噬细胞在妊娠子宫中具有重要的抑制功能。
PROBLEM: Local immune modulation has been shown to be of considerable importance for the maintenance of successful pregnancy. We have previously reported the secretion of interferon-gamma (IFN-gamma), interleukin-4 (IL-4) and IL-10 in human decidua from early normal pregnancy. The aim of this study was to investigate the cellular source of cytokine secretion in the decidua, and compare this to secretion patterns in peripheral blood.METHOD OF STUDY: Decidual tissue and peripheral blood was collected from 20 women undergoing surgical abortion during first trimester pregnancy. Monocytes/macrophages and NK cells were enriched by immunomagnetic cell separation and cytokine secretion was detected by enzyme-linked immunosorbent spot-forming cell assay.RESULTS: Decidual and peripheral monocytes/macrophages and NK cells spontaneously secrete IFN-gamma, IL-4 and IL-10. The number of IL-10 secreting cells was significantly higher in decidual macrophages compared with decidual non-monocytic cells as well as compared with blood monocytes/macrophages. These differences were not seen for IFN-gamma or IL-4.CONCLUSIONS: Our results indicate that decidual macrophages subserve important suppressive functions in the pregnant uterus.