Brief bout of exercise alters gene expression in peripheral blood mononuclear cells of early- and late-pubertal males.
Brief bout of exercise alters gene expression in peripheral blood mononuclear cells of early- and late-pubertal males.
复制标题
DOI：
10.1203/pdr.0b013e3181993473
复制
发表时间：
2009-04
影响因子：
3.6
通讯作者：
Cooper DM
中科院分区：
文献类型：
--
作者：
Radom-Aizik S;Zaldivar F Jr;Leu SY;Cooper DM
外周血单核细胞 (PBMC) 受到运动的刺激，不仅有助于宿主防御，还有助于生长、修复和疾病发病机制。儿童运动是否会改变 PBMC 基因表达尚不清楚。 10 名青春期早期男孩（8-12 岁）和 10 名青春期晚期男孩（15-18 岁）进行了 10 次 2 分钟的剧烈、恒定工作强度运动，中间休息 1 分钟。在运动前后分离PBMC并进行微阵列（Affymetrix U133+2芯片）分析。识别基因表达变化的统计标准是错误发现率低于 5%，置信度为 95%。在年龄较大的男孩中，有 1246 个基因发生了改变（517 个向上，729 个向下），但在年轻组中只有 109 个基因发生改变（79 个向上，30 个向下）。在年龄较大的男孩中，发现了 13 条基因途径（使用 EASE p<0.05）（例如自然杀伤细胞细胞毒性、细胞凋亡）。上皮调节蛋白基因表达（EREG，一种参与伤口愈合的生长因子）在年龄较大的男孩中增加。在年龄较大的男孩中，运动改变了 TBX21、GZMA、PGTDR 和 CCL5 等基因，这些基因也在哮喘等儿科炎症性疾病中发挥作用。两组中有 66 个基因发生显着变化。 PBMC 基因表达模式表明与能量消耗突然变化相关的免疫“危险”信号的启动。
Peripheral blood mononuclear cells (PBMCs) are stimulated by exercise and contribute not only to host defense, but also to growth, repair, and disease pathogenesis. Whether PBMC gene expression is altered by exercise in children is not known. 10 early pubertal boys (8-12 y) and 10 late pubertal boys (15-18 y) performed 10, 2-min bouts of strenuous, constant work rate exercise with 1-min rest intervals. PBMCs were isolated before and after exercise and microarray (Affymetrix U133+2 chips) analyzed. Statistical criterion to identify gene expression changes was less than 5% false discovery rate with 95% confidence. 1246 genes were altered in older boys (517 up, 729 down), but only 109 in the younger group (79 up, 30 down). In older boys, 13 gene pathways (using EASE p<0.05) were found (e.g., natural killer cell cytoxicity, apoptosis). Epiregulin gene expression (EREG, a growth factor involved in wound healing) increased in older boys. In older boys exercise altered genes such as TBX21, GZMA, PGTDR, and CCL5 that also play roles in pediatric inflammatory diseases like asthma. 66 genes changed significantly in both groups. The pattern of PBMC gene expression suggests the initiation of an immunological “danger” signal associated with a sudden change in energy expenditure.