Accelerating Turnover Frequency in Nucleic Acid Templated Reactions
Accelerating Turnover Frequency in Nucleic Acid Templated Reactions
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DOI：
10.1021/acs.bioconjchem.7b00663
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发表时间：
2018-01-01
影响因子：
4.7
通讯作者：
Winssinger, Nicolas
中科院分区：
文献类型：
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作者：
Chang, Dalu;Kim, Ki Tae;Winssinger, Nicolas
核酸模板化反应作为检测寡核苷酸和将基于核酸的指令翻译成不同输出的重要技术已经引起关注。在加速反应以改善信号放大方面已经取得了很大进展，达到了底物周转而不是化学反应限制速率的速率。在此，我们探索了受三向连接启发的架构的实用性，该三向连接产生链的切割，从而加速底物周转。我们证明，这样的设计可以克服模板化反应中的产物抑制，并接近杂交的速率进行操作。
Nucleic acid templated reactions have attracted attention as an important technology to sense oligonucleotides and to translate nucleic acid-based instructions into diverse outputs. Great progress has been made in accelerating the reaction in order to improve signal amplification, reaching rates where substrate turnover rather than chemical reaction is rate limiting. Herein we explore the utility of architectures inspired by three-way junction that yield a cleavage of a strand thus accelerating substrate turnover. We demonstrate that such design can overcome product inhibition in templated reactions and operate close to the rate of hybridization.