MECHANISM AND CATALYSIS OF REACTIONSOF ACYL PHOSPHATES .2. HYDROLYSIS
MECHANISM AND CATALYSIS OF REACTIONSOF ACYL PHOSPHATES .2. HYDROLYSIS
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DOI：
10.1021/ja01482a025
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发表时间：
1961-01-01
影响因子：
15
通讯作者：
JENCKS, WP
中科院分区：
文献类型：
--
作者：
DISABATO, G;JENCKS, WP
结果乙酰磷酸一价阴离子（AcP-）和二价阴离子（AcP-）的水解速率见表I;在39 ℃下，0.6 Mkc ~（-1）的水解速率与Lynen 14和Koshland的结果一致。单阴离子和双阴离子水解的速率在溶剂氧化氘中没有显著改变，并且在浓KCl、Na-Cl 〇 4和NaCl中没有改变或略有增加。该速率对三羟甲基氨基甲烷（TRIS）缓冲液的浓度不敏感，与浓磷酸盐、醋酸盐或砷酸盐缓冲液不发生显着反应或共离子效应。在30%和50%乙腈中，ACP-水解速率不变，而ACP-水解速率略有下降。还记录了在0、25和50下的水解速率以及导出的f * 和AS值。乙酰苯基磷酸盐的水解速率随着H2O和D2 O中的磷酸盐缓冲液浓度的增加而增加（图1）。的de-as）S. Hestrin，J. Biol. Chem.，180，249（1949）。
Results The rates of acetyl phosphate monoanion (AcP-) and dianion (AcP-) hydrolysis are summarized in Table I; the rates in 0.6 Mkc1 at 39 agree satis-factorily with those previously obtained by Lynen14 and by Koshland. 15· 16 The rates of monoanion and dianion hydrolysis are not significantly altered in solvent deuterium oxide and are not changed or are slightly increased by concentrated KC1, Na-C104 and NaCl. The rates are also insensitive to the concentration of tris-(hydroxymethyl)-amino-methane (TRIS) buffer and donot show a signifi-cant reaction or common ion effect with concen-trated phosphate, acetate or arsenate buffers. In 30 and 50% acetonitrile the rate of AcP-hydrolysis is unchanged and that of AcP-is slightly decreased. The rates of hydrolysis at 0, 25 and 50 and the derived//* and AS-values also are recorded. The rate of hydrolysis of acetyl phenyl phosphate increases with increasing concentrations of phos-phate buffer in H20 and D20 (Fig. 1). The de-as) S. Hestrin, J. Biol. Chem., 180, 249 (1949).