Simulation of heat exposure and damage to the eye lens in a neighborhood bakery
Simulation of heat exposure and damage to the eye lens in a neighborhood bakery
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DOI：
10.1016/j.exer.2008.04.007
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发表时间：
2008-07-01
影响因子：
3.4
通讯作者：
Dovrat, Ahuva
中科院分区：
文献类型：
--
作者：
Sharon, Naomi;Bar-Yoseph, Pinhas Z.;Dovrat, Ahuva
流行病学研究表明，高温环境与白内障之间存在联系。这项研究的目的是调查附近面包店的高温是否会对眼睛透镜造成损害。进行测量以确定工作日期间附近面包店的温度和暴露时间。使用有限体积和有限元计算流体动力学（CFD）代码进行热分析，以确定暴露于环境温度波动时眼睛透镜中的温度。使用牛透镜器官培养系统进行热暴露的模拟。将270枚牛晶状体分为5组。(1)对照组保持在培养物中11-14天（2）镜片暴露于39.5 ℃，从培养的第二天开始每天6小时，并保持在培养物中13天（3）镜片暴露于39.5 ℃，从培养的第二天开始每天4小时，并保持在培养物中11天（4）镜片暴露于39.5 ℃，从培养的第二天开始，每天2小时，持续3天，并保持培养12天（5）将镜片暴露于39.5 ℃，在培养的第二天1小时，并保持培养14天。在培养期间评估透镜光学质量。在培养结束时，通过倒置显微镜证实透镜损伤。取透镜上皮样品用于过氧化氢酶活性分析。对照镜片在整个14天的培养中保持其光学质量。暴露于高温对培养的晶状体造成光学损伤。6 h暴露组损伤出现较早，并从透镜前缝向其中心发展。在暴露于39.5 ℃ 1 h的晶状体中，光学损伤得到恢复，但损伤仍存在于透镜上皮细胞中。我们的研究表明，在面包房里暴露于高温会对眼睛透镜造成损害，这种损害取决于暴露的时间长短。(C)2008爱思唯尔有限公司保留所有权利。
Epidemiological studies indicated a link between high temperature environment and cataract. The purpose of the study was to investigate if the high temperature in neighborhood bakeries can cause damage to the eye lens. Measurements were done to determine the temperature and exposure time in the neighborhood bakeries during a workday. Thermal analysis was done using finite volume and finite element Computational Fluid Dynamics (CFD) codes in order to determine the temperature in the eye lens when exposed to environmental temperature fluctuations. A simulation of heat exposure was carried out using a bovine lens organ culture system. Two-hundred and seventy bovine lenses were divided into five groups. (1) Control group kept in culture for 11-14 days (2) Lenses exposed to 39.5 degrees C, 6 h daily starting on the second day of the culture and kept in culture for 13 days (3) Lenses exposed to 39.5 degrees C, 4 h daily starting on the second day of the culture and kept in culture for 11 days (4) Lenses exposed to 39.5 degrees C, 2 h daily for 3 days starting on the second day of the culture and kept in culture for 12 days (5) Lenses exposed to 39.5 degrees C, 1 h on the second day of the culture and kept in culture for 14 days. Lens optical quality was assessed during the culture period. At the end of the culture lens damage was demonstrated by inverted microscopy. Lens epithelial samples were taken for analysis of Catalase activities. Control lenses maintained their optical quality throughout the 14 days of the culture. Exposure to heat caused optical damage to the cultured lenses. The damage appeared earlier in the 6 h exposure group and progressed from the lens anterior suture to its center. Optical damage was recovered in lenses exposed 1 h to 39.5 degrees C, but the damage remained in the lens epithelial cells. Our study indicates that exposure to heat in bakeries can cause damage to the eye lens and that the damage is dependent on the length of exposure. (C) 2008 Elsevier Ltd. All rights reserved.