Collagen-hyaluronic acid scaffolds for adipose tissue engineering
Collagen-hyaluronic acid scaffolds for adipose tissue engineering
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DOI：
10.1016/j.actbio.2010.05.005
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发表时间：
2010-10-01
影响因子：
9.7
通讯作者：
Cameron, R. E.
中科院分区：
文献类型：
--
作者：
Davidenko, N.;Campbell, J. J.;Cameron, R. E.
乳腺Intro模型中的三维（3-D）模型需要一种支架基质，可以支持脂肪基质在坚固的自由渗透基质中发育。用1-乙基-3-（3-二甲氨基丙基）-碳二亚胺盐酸盐交联，采用可控冷冻干燥技术制备了胶原-透明质酸（HA-75%和15%）三维多孔支架。热特性的值表明，交联发生，其效率提高了HA的存在下，虽然交联减少了在水中的膨胀的支柱材料，胶原-HA基质作为一个整体往往膨胀更多，并显示出更高的耐溶解性比纯胶原样品的压缩模量和弹性塌陷应力更高的胶原-HA复合材料。所有支架均显示支持3 T3-L1前脂肪细胞的增殖和分化，而胶原-HA样品保持了显著增加的循环细胞（K1-67+）比例。此外，胶原-HA复合物显示出与对照条件相比，在脂肪形成培养物补充8天的情况下显著提高的Adipsin基因表达。这些结果表明，胶原蛋白-HA支架可以提供稳健的、自由渗透的3-D基质，其增强体外乳腺基质组织发育。版权所有（C）2010由Elsevier Ltd代表Acta Materialia Inc出版。保留所有权利。
Three-dimensional (3-D) in Intro models of the mammary gland require a scaffold matrix that supports the development of adipose stroma within a robust freely permeable matrix. 3-D porous collagen-hyaluronic acid (HA- 7 5% and 15%) scaffolds were produced by controlled freeze-drying technique and crosslinking with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride All scaffolds displayed uniform, interconnected pore structure (total porosity similar to 85%) Physical and chemical analysis showed no signs of collagen denaturation during the formation process. The values of thermal characteristics indicated that crosslinking occurred and that its efficiency was enhanced by the presence of HA Although the crosslinking reduced the swelling of the strut material in water, the collagen-HA matrix as a whole tended to swell more and show higher dissolution resistance than pure collagen samples The compressive modulus and elastic collapse stress were higher for collagen-HA composites. All the scaffolds were shown to support the proliferation and differentiation 3T3-L1 preadipocytes while collagen-HA samples maintained a significantly increased proportion of cycling cells (K1-67+). Furthermore, collagen-HA composites displayed significantly raised Adipsin gene expression with adipogenic culture supplementation for 8 days vs control conditions. These results indicate that collagen-HA scaffolds may offer robust, freely permeable 3-D matrices that enhance mammary stromal tissue development in vitro Crown Copyright (C) 2010 Published by Elsevier Ltd on behalf of Acta Materialia Inc All rights reserved.