Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays
Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays
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DOI：
10.1016/j.bioelechem.2018.01.009
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发表时间：
2018-06-01
影响因子：
5
通讯作者：
Barbosa, Rui M.
中科院分区：
文献类型：
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作者：
Ferreira, Nuno R.;Ledo, Ana;Barbosa, Rui M.
纳米复合材料传感器由Nafion和碳纳米管修饰的碳纤维微电极和基于陶瓷的微电极生物传感器阵列组成，用于以高空间，时间和化学分辨率测量大脑中的抗坏血酸和谷氨酸。纳米复合材料传感器显示对抗坏血酸氧化的电催化性能，转化为从+0.20 V到-0.05 V的负移（相对于Ag/AgCl），以及电活性表面积的显着增加（10倍）。在海马的CA 1、CA 3和齿状回（DG）亚区中，体内抗坏血酸盐的估计平均基础浓度分别为276 +/- 60 μ M（n = 10）、183 +/- 30 μ M（n = 10）和133 +/- 42 μ M（n = 10）。谷氨酸微生物传感器阵列显示出5.3 +/- 0.8 pA pM（-1）（n = 18）的高灵敏度，204 +/- 32 nM（n = 10）的LOD，和0.9 +/- 0.02 s（n = 6）的t（50%）响应时间和对主要干扰物的高选择性。同时和实时测量的谷氨酸和抗坏血酸在麻醉大鼠的海马局部刺激后，KCl或谷氨酸揭示了两个神经化学物质之间的动态相互作用。(C)2018爱思唯尔B. V.保留所有权利。
Nanocomposite sensors consisting of carbon fiber microelectrodes modified with Nafion and carbon nanotubes, and ceramic-based microelectrode biosensor arrays were used to measure ascorbate and glutamate in the brain with high spatial, temporal and chemical resolution. Nanocomposite sensors displayed electrocatalytic properties towards ascorbate oxidation, translated into a negative shift from +0.20 V to -0.05 V vs. Ag/AgCl, as well as a significant increase (10-fold) of electroactive surface area. The estimated average basal concentration of ascorbate in vivo in the CA1, CA3 and dentate gyrus (DG) sub regions of the hippocampus were 276 +/- 60 mu M (n = 10), 183 +/- 30 mu M (n = 10) and 133 +/- 42 mu M (n = 10), respectively. The glutamate microbiosensor arrays showed a high sensitivity of 5.3 +/- 0.8 pA pM(-1) (n = 18), and LOD of 204 +/- 32 nM (n = 10), and t(50%) response time of 0.9 +/- 0.02 s (n = 6) and high selectivity against major interferents. The simultaneous and real-time measurements of glutamate and ascorbate in the hippocampus of anesthetized rats following local stimulus with KCl or glutamate revealed a dynamic interaction between the two neurochemicals. (C) 2018 Elsevier B.V. All rights reserved.