Effect of cinnamon, clove and some of their constituents on the Na+-K+-ATPase activity and alanine absorption in the rat jejunum
Effect of cinnamon, clove and some of their constituents on the Na+-K+-ATPase activity and alanine absorption in the rat jejunum
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DOI：
10.1016/s0278-6915(00)00073-9
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发表时间：
2000-09-01
影响因子：
4.3
通讯作者：
Copti, R
中科院分区：
文献类型：
--
作者：
Kreydiyyeh, SI;Usta, J;Copti, R
本文研究了几种香辛料水提液对离体大鼠空肠Na ~+-K ~+-ATP酶活性的影响。肉豆蔻、肉桂、茴香、小茴香、芫荽、姜黄和香菜的提取物均对ATP酶有抑制作用，而八角和白色胡椒的作用不明显。与其他香料提取物相比，桂皮和肉桂提取物对肠道ATP酶的抑制作用最强。它们还抑制粗肾匀浆中的体外Na ~+-K ~+-ATP酶活性和离体狗肾Na ~+-K ~+-ATP酶活性。肉桂醇提物对空肠酶的抑制作用较水提物强，IC_（50）值较低，与肉桂醛（肉桂挥发油的主要成分）无显著差异，表明肉桂醛是肉桂醇提物的主要抑制成分。丁香酚、丁香水提取物和丁香乙醇提取物的IC 50值均落在相同范围内，并且彼此无显著差异，表明丁香酚是乙醇和水提取物中的主要抑制组分。根据IC_（50）值，该酶对香辛料提取物的敏感性顺序为：离体狗肾ATP酶>大鼠肾ATP酶>大鼠肠ATP酶。桂皮和肉桂的水提取物也显著降低了大鼠肠道对丙氨酸的吸收。结果表明，桂皮和肉桂中的有效成分能透过肠上皮细胞膜，抑制Na+-K+-ATP酶，而Na+-K+-ATP酶是许多转运过程的驱动力。(C)2000爱思唯尔科技有限公司版权所有。
The effect of a water extract of some spices on the in vitro activity of the rat jejunal Na+-K+-ATPase was investigated. Extracts of nutmeg, cinnamon, clove, cumin, coriander, turmeric and caraway all inhibited the ATPase, while aniseed and white pepper exerted no significant effects. The extracts of clove and cinnamon had the most potent inhibitory effect on the intestinal ATPase as compared to extracts of other spices. They also inhibited the in vitro Na+-K+-ATPase activity in a crude kidney homogenate and the activity of an isolated dog kidney Na+-K+-ATPase. The alcoholic extract of cinnamon, compared to the aqueous extract, had a stronger inhibitory action on the jejunal enzyme and a lower IC50 value, which was not significantly different from the one observed with cinnamaldehyde, the major volatile oil present cinnamon, suggesting that in alcoholic extracts cinnamaldehyde is the major inhibitory component. The IC50 values of eugenol, aqueous clove extract and ethanolic clove extract all fell within the same range and were not significantly different from each other, suggesting that eugenol is the major inhibitory component in both alcoholic and aqueous extracts. Based on the IC50 values, the order of sensitivity of the enzyme to the spices extracts is as follows: isolated dog kidney ATPase > rat kidney ATPase > rat intestine ATPase. The aqueous extracts of clove and cinnamon also significantly lowered the absorption of alanine from the rat intestine. It was concluded that the active principle(s) in clove and cinnamon can permeate the membrane of the enterocytes and inhibit the Na+-K+-ATPase that provides the driving force for many transport processes. (C) 2000 Elsevier Science Ltd. All rights reserved.