SITE-RELATED VARIATIONS IN GLYCOSAMINOGLYCAN CONTENT AND SWELLING PROPERTIES OF BOVINE FLEXOR TENDON
SITE-RELATED VARIATIONS IN GLYCOSAMINOGLYCAN CONTENT AND SWELLING PROPERTIES OF BOVINE FLEXOR TENDON
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DOI：
10.1002/jor.1100050314
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发表时间：
1987-01-01
影响因子：
2.8
通讯作者：
VOGEL, KG
中科院分区：
文献类型：
--
作者：
KOOB, TJ;VOGEL, KG
成年牛深屈肌腱内存在形态学上不同的组织提出了一个模型，我们研究了蛋白多糖含量和组织肿胀特性之间的相关性。仅经受张力的近端部分含有低水平的糖胺聚糖（占干重百分比为0.2%的糖胺聚糖氨基己糖），其均匀分布在整个长度和厚度上。胶原蛋白占组织质量的80%，胶原纤维形成平行阵列，纵向拉伸力的方向。在平衡体积膨胀试验中，近端组织的性质在整个组织中是均匀的，并且是胶原组织的典型性质，其中对低离子强度缓冲液或酸性pH中的平衡的响应由胶原网络主导。远端深屈肌腱的前面与僵硬的腱旁鞘和籽骨连接，除了纵向拉力外，还受到压缩力和摩擦力。沿着该前表面并部分延伸到肌腱中的是类似于纤维软骨的组织，其具有组织成随机网络的胶原纤维。最大的糖胺聚糖氨基己糖含量（干重的2-3%）被发现在远端表层，和大的蛋白聚糖集中在网站接收直接的压缩和摩擦负荷。远端组织的平衡体积膨胀试验表明，关节面层具有独特的材料特性。当反离子从组织中冲洗时，远端表面层膨胀，而降低的pH值对远端组织体积几乎没有影响。远端组织的肿胀特性与蛋白多糖含量直接相关，并且与关节软骨的肿胀特性相似。这些结果表明，远端屈肌腱的关节层适合于一组独特的机械要求，并且在压缩力和摩擦力的部位蛋白多糖含量升高有助于满足这些功能需求。
The presence of morphologically distinct tissues within the adult bovine deep flexor tendon presented a model which we examined for correlations between proteoglycan content and tissue swelling properties. The proximal portion which experiences only tensile forces contained low levels of glycosaminoglycan (0.2% glycosaminoglycan hexosamine as percentage of dry weight) that were evenly distributed throughout its length and thickness. Collagen accounted for 80% of tissue mass, and collagen fibers formed parallel arrays running longitudinal to the direction of tensile force. In equilibrium bulk swelling tests, the properties of proximal tissue were uniform throughout the tissue and typical of collagenous tissues in which the response to equilibration in low ionic strength buffers or acid pH is dominated by the collagen network. The anterior aspect of the distal deep flexor tendon articulates with the stiff paratendinous sheath and sesamoid bones and is subjected to compressive and frictional forces in addition to longitudinal tensile forces. Along this anterior surface and extending partially into the tendon is tissue that resembles fibrocartilage with collagen fibers organized as a random network. Greatest glycosaminoglycan hexosamine contents (2-3% of dry weight) were found in the distal surface layer, and large proteoglycans were concentrated at the site receiving direct compressive and frictional loads. Equilibrium bulk swelling tests on distal tissue showed that the articulating surface layer possessed unique material properties. Distal surface layers swelled when counter ions were washed from the tissue, whereas lowered pH had little effect on distal tissue volume. Swelling properties of distal tissue correlated directly with proteoglycan content and were similar to swelling properties of articular cartilage. These results suggest that the articulating layer of distal flexor tendon is adapted for a unique set of mechanical requirements and that an elevated proteoglycan content at the site of compressive and frictional forces contributes to meeting these functional needs.