Correlation of cell-mediated immunity against porcine reproductive and respiratory syndrome virus with protection against reproductive failure in sows during outbreaks of porcine reproductive and respiratory syndrome in commercial herds
Correlation of cell-mediated immunity against porcine reproductive and respiratory syndrome virus with protection against reproductive failure in sows during outbreaks of porcine reproductive and respiratory syndrome in commercial herds
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DOI：
10.2460/javma.2005.226.1707
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发表时间：
2005-05-15
影响因子：
1.9
通讯作者：
Goldberg, TL
中科院分区：
文献类型：
--
作者：
Lowe, JF;Husmann, R;Goldberg, TL
试验-确定抗猪繁殖与呼吸综合征（PRRS）病毒的细胞介导免疫是否与商业猪群中PRRS临床爆发期间母猪繁殖失败的保护相关。设计-在4个种猪群中进行爆发调查。动物-97头母猪。程序-在每个农场，从具有临床体征的母猪中采集血样（流产或胎死增加，病例母猪）和临床正常母猪（对照母猪）。通过使用干扰素-γ酶联免疫斑点（ELISPOT）试验测定细胞介导免疫（CMI）应答的强度。使用多元逻辑回归分析和t检验比较病例母猪和对照母猪之间的ELISPOT测定值。多元线性回归被用来调查细胞介导的免疫和临床signs.Results-In 2农场的幅度之间的关联，情况母猪ELISPOT测定值低于对照母猪。在1个农场检测到CMI反应强度与每窝出生死亡猪数之间存在负相关。在1个农场，没有关联检测到的CMI反应和保护生殖failure.Conclusions和临床相关性的证据，一个强大的CMI反应与保护临床PRRS之间的强度检测在3 4农场。然而，猪场和猪场内母猪的免疫反应和临床保护程度差异很大。增加感染畜群内细胞介导的免疫力有可能减少临床生殖疾病，但前提是可以确定PRRS病毒细胞介导免疫力强度的农场内和农场间变异来源。
Objective-To determine whether cell-mediated immunity against porcine reproductive and respiratory syndrome (PRRS) virus is correlated with protection against reproductive failure in sows during clinical outbreaks of PRRS in commercial herds.Design-Outbreak investigation in 4 swine breeding herds.Animals-97 sows.Procedures-On each farm, blood samples were collected from sows with clinical signs (abortion or increased fetal death, case sows) and from clinically normal sows (control sows). The intensity of the cell-mediated immune (CMI) response was determined by use of an intederon-γ enzyme-linked immunospot (ELISPOT) assay. Multiple logistic regression analyses and t tests were used to compare ELISPOT assay values between case and control sows. Multiple linear regression was used to investigate associations between cell-mediated immunity and the magnitude of clinical signs.Results-In 2 farms, case sows had lower ELISPOT assay values than control sows. A negative association between the intensity of the CMI response and the number of pigs born dead per litter was detected on 1 farm. In 1 farm, no association was detected between the intensity of the CMI response and protection against reproductive failure.Conclusions and Clinical Relevance-Evidence that a strong CMI response was correlated with protection against clinical PRRS was detected in 3 of 4 farms. However, farms and sows within farms varied considerably in their immune responsiveness and in the degree to which they were protected clinically. Increasing cell-mediated immunity within infected herds has the potential to decrease clinical reproductive disease, but only if the sources of intra- and inter-farm variation in the intensity of cell-mediated immunity to PRRS virus can be identified.