Assimilation of Formaldehyde in Transgenic Plants Due to the Introduction of the Bacterial Ribulose Monophosphate Pathway Genes
Assimilation of Formaldehyde in Transgenic Plants Due to the Introduction of the Bacterial Ribulose Monophosphate Pathway Genes
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DOI：
10.1271/bbb.90847
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发表时间：
2010-03-01
影响因子：
1.6
通讯作者：
Izui, Katsura
中科院分区：
文献类型：
--
作者：
Chen, Li-mei;Yurimoto, Hiroya;Izui, Katsura
甲醛（HCHO）是一种空气污染物，被怀疑是致癌的，也是引起病室综合症的原因。被称为甲基营养菌的微生物可以利用还原的C-1化合物，如甲烷和甲醇，固定和同化HCHO，而大多数植物不能直接同化HCHO。我们发现，通过基因工程，细菌的甲酰胺固定途径（核酮糖单磷酸途径）可以整合作为一个旁路的Calvin-Benson循环在转基因拟南芥和烟草。这些植物表现出对甲醛的耐受性增强，并且通过将其固定为糖磷酸盐来消除气态甲醛的能力增强。我们的研究结果提供了一种新的策略，为植物修复甲醛污染，也代表了第一步生产的植物，可以同化天然气衍生的C-1化合物。
Formaldehyde (HCHO) is an air pollutant suspected of being carcinogenic and a cause of sick-house syndrome. Microorganisms called methylotrophs, which can utilize reduced C-1 compounds such as methane and methanol, fix and assimilate HCHO, whereas most plants are unable to assimilate HCHO directly. We found that a bacterial formaldehyde-fixing pathway (ribulose monophosphate pathway) can be integrated as a bypass to the Calvin-Benson cycle in transgenic Arabidopsis thaliana and tobacco by genetic engineering. These plants showed enhanced tolerance to HCHO and enhanced capacity to eliminate gaseous HCHO by fixing it as a sugar phosphate. Our results provide a novel strategy for phytoremediation of HCHO pollution, and also represent the first step toward the production of plants that can assimilate natural gas-derived C-1 compounds.