An aptamer-targeting photoresponsive drug delivery system using "off-on" graphene oxide wrapped mesoporous silica nanoparticles.
An aptamer-targeting photoresponsive drug delivery system using "off-on" graphene oxide wrapped mesoporous silica nanoparticles.
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使用“关闭”氧化石墨烯包裹介孔二氧化硅纳米粒子的适体靶向光响应药物递送系统
DOI：
10.1039/c4nr07493a
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发表时间：
2015-04-14
期刊：
影响因子：
6.7
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Tang Y;Hu H;Zhang MG;Song J;Nie L;Wang S;Niu G;Huang P;Lu G;Chen X
我们开发了一种新型的适体靶向光响应药物递送系统，通过在氧化石墨烯包裹的多柔比星（Dox）负载的介孔二氧化硅纳米颗粒（MSN-Dox@GO-Apt）表面非共价组装Cy 5. 5-AS 1411适体缀合物，用于光介导的药物释放和适体靶向癌症治疗。MSN-Dox@GO-Apt的两个“关-开”开关分别由适体靶向和光触发控制。Cy5.5-AS 1411配体通过“关-开”Cy5.5荧光恢复为MSN-Dox@GO-Apt提供核仁素特异性靶向和实时指示剂能力。GO充当看门人，以防止装载的Dox在没有激光照射的情况下泄漏，并控制Dox响应于激光照射的释放。当GO包裹福尔斯在激光照射时脱落时，“关-开”光响应药物递送系统被激活，从而诱导化疗。有趣的是，随着激光功率的增加，化疗和光热疗法在单一MSN-Dox@GO-Apt平台中的协同作用导致比单一疗法更有效的癌细胞杀伤，为癌症治疗提供了一种新方法。
We have developed a novel aptamer-targeting photoresponsive drug delivery system by non-covalent assembly of a Cy5.5-AS1411 aptamer conjugate on the surface of graphene oxide wrapped doxorubicin (Dox)-loaded mesoporous silica nanoparticles (MSN-Dox@GO-Apt) for light-mediated drug release and aptamer-targeted cancer therapy. The two “off–on” switches of the MSN-Dox@GO-Apt were controlled by aptamer targeting and light triggering, respectively. The Cy5.5-AS1411 ligand provides MSN-Dox@GO-Apt with nucleolin specific targeting and real-time indicator abilities by “off–on” Cy5.5 fluorescence recovery. The GO acts as a gatekeeper to prevent the loaded Dox from leaking in the absence of laser irradiation, and to control the Dox release in response to laser irradiation. When the GO wrapping falls off upon laser irradiation, the “off–on” photoresponsive drug delivery system is activated, thus inducing chemotherapy. Interestingly, with an increase in laser power, the synergism of chemotherapy and photothermal therapy in a single MSN-Dox@GO-Apt platform led to much more effective cancer cell killing than monotherapies, providing a new approach for treatment against cancer.