Hierarchical-Multiplex DNA Patterns Mediated by Polymer Brush Nanocone Arrays That Possess Potential Application for Specific DNA Sensing.
Hierarchical-Multiplex DNA Patterns Mediated by Polymer Brush Nanocone Arrays That Possess Potential Application for Specific DNA Sensing.
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DOI：
10.1021/acsami.5b07577
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发表时间：
2015-11
影响因子：
9.5
通讯作者：
Wendong Liu;Xueyao Liu;Peng Ge;L. Fang;Siyuan Xiang;Xiaohuan Zhao;Huaizhong Shen;Bai Yang
中科院分区：
文献类型：
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作者：
Wendong Liu;Xueyao Liu;Peng Ge;L. Fang;Siyuan Xiang;Xiaohuan Zhao;Huaizhong Shen;Bai Yang
利用聚甲基丙烯酸羟乙酯（PHEMA）刷作为载体，制备了单链DNA（ssDNA）纳米锥阵列和层次DNA图案。采用胶体光刻法制备了不同形貌和周期的PHEMA刷状纳米锥阵列。采用胶体光刻和传统光刻相结合的方法制备了分级结构。通过将氨基修饰的单链DNA接枝到聚合物刷的侧链上，可以容易地获得DNA图案，并且锚定的DNA保持其反应性。所制备的ssDNA纳米锥阵列可用于靶DNA传感，检测限达到1.65 nM。此外，通过引入微流控技术，可以在同一基底上实现多级复合DNA图案，每种图案都含有一种ssDNA，这为制备快速、可见、复合的DNA传感器提供了良好的表面条件。
This paper provides a facile and cost-efficient method to prepare single-strand DNA (ssDNA) nanocone arrays and hierarchical DNA patterns that were mediated by poly(2-hydroxyethyl methacrylate) (PHEMA) brush. The PHEMA brush nanocone arrays with different morphology and period were fabricated via colloidal lithography. The hierarchical structure was prepared through the combination of colloidal lithography and traditional photolithography. The DNA patterns were easily achieved via grafting the amino group modified ssDNA onto the side chain of polymer brush, and the anchored DNA maintained their reactivity. The as-prepared ssDNA nanocone arrays can be applied for target DNA sensing with the detection limit reaching 1.65 nM. Besides, with the help of introducing microfluidic ideology, the hierarchical-multiplex DNA patterns on the same substrate could be easily achieved with each kind of pattern possessing one kind of ssDNA, which are promising surfaces for the preparation of rapid, visible, and multiplex DNA sensors.