Electronic Supplementary Material ( ESI ) for Soft Matter
Electronic Supplementary Material ( ESI ) for Soft Matter
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发表时间：
2014
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影响因子：
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通讯作者：
Rachel Baarda;Tegan L. Marianchuk;Michael D. Toney
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文献类型：
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作者：
Rachel Baarda;Tegan L. Marianchuk;Michael D. Toney
通过电铸法制备GUV基于Okamura等1的方案如下修改。在灌注室盖孔的底部制作三组相距2 mm的切口，以容纳两根铂丝和一根直径为50-100μm的窄玻璃毛细管。用氯仿中的0.1M脂质溶液（所需脂质混合物）薄层涂覆玻璃毛细管，并使其干燥几分钟。将灌注室盖孔TM置于成像间隔物安全密封件的粘合剂表面上，然后将其置于显微镜载玻片上并使其粘附数分钟。然后用MilliQ水填充腔室，确保在Pt线或玻璃毛细管周围没有形成气泡。使用鳄鱼夹将铂丝连接到在10 Hz和2 V下运行的函数发生器（内部构建）。电铸通常进行2小时。
Preparation of GUVs by and electroformation method A protocol based on Okamura et al.1 was adapted as follows. Three sets of incisions, 2mm apart, were made on the bottom of a Perfusion Chamber Cover Well to accommodate two platinum wires and a narrow glass capillary, 50-100μm in diameter. The glass capillary was coated with a thin layer of a 0.1M lipid solution (of the required lipid mixture) in chloroform, and allowed to dry for several minutes. A perfusion Chamber Cover Well TM was placed onto the adhesive surface of an imaging Spacer Secure-Seal, which was in turn placed onto a microscope slide and allowed to adhere for several minutes. The chamber then was filled with MilliQ water ensuring no air bubbles were formed around the Pt wires or the glass capillary. The platinum wires were connected to a function generator (built in-house) operating at 10Hz and 2V using crocodile clips. Electroformation was carried out typically for two hours.