Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy Kashin-Beck Disease.
Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy Kashin-Beck Disease.
复制标题
地方性骨软骨病大骨节病软骨中霉菌毒素相关基因表达谱分析
DOI：
10.1186/1471-2474-13-130
复制
发表时间：
2012-07-24
影响因子：
2.3
通讯作者：
Yan H
中科院分区：
文献类型：
--
作者：
Zhang F;Guo X;Wang W;Wu S;Ma W;Yan H
背景大骨节病（KBD）是一种地方性骨软骨病。真菌毒素被认为在大骨节病的发病机制中起重要作用。由于真菌毒素引起软骨损伤的分子机制尚不清楚，目前尚无有效的治疗方法。为明确霉菌毒素诱导软骨病变的关键基因，比较大骨节病软骨和健康软骨中霉菌毒素相关基因（mycotoxin-related genes，MRG）的表达谱。进行qRT-PCR以验证微阵列数据。1，167个MRG来自环境相关基因组数据库Toxicogenomics。结果在大骨节病软骨组织中发现7个上调表达的MRG和2个下调表达的MRG，它们与胶原蛋白、细胞凋亡、代谢和生长发育有关。GO表达分析发现，大骨节病软骨中4个病程相关GO和5个生长发育相关GO表达显著上调，提示真菌毒素可能通过影响软骨中胶原、细胞凋亡和生长发育相关MRG功能而参与大骨节病的发生发展。
BackgroundKashin-Beck Disease (KBD) is an endemic osteochondropathy. Mycotoxins are believed to play an important role in the pathogenesis of KBD. Because the molecular mechanism of mycotoxin-induced cartilage lesions remains unclear, there is not effective treatment for KBD now. To identify key genes involved in the mycotoxin-induced cartilage lesions, we compared the expression profiles of mycotoxin-related genes (MRG) between KBD cartilage and healthy cartilage.MethodsTotal RNA was isolated from cartilage samples, following by being amplified, labeled and hybridized to Agilent human whole genome microarray chip. qRT-PCR was conducted to validate the microarray data. 1,167 MRG were derived from the environmentally related genomic database Toxicogenomics. The microarray data of MRG was subjected to single gene and gene ontology (GO) expression analysis for identifying differently expressed genes and GO.ResultsWe identified 7 up-regulated MRG and 2 down-regulated MRG in KBD cartilage, involved in collagen, apoptosis, metabolism and growth & development. GO expression analysis found that 4 apoptosis-related GO and 5 growth & development-related GO were significantly up-regulated in KBD cartilage.ConclusionsBased on the results of previous and our studies, we suggest that mycotoxins might contribute to the development of KBD through dysfunction of MRG involved in collagen, apoptosis and growth & development in cartilage.
登录
查看更多内容
影响因子：
4.5
作者：
Downey CM;Horton CR;Carlson BA;Parsons TE;Hatfield DL;Hallgrímsson B;Jirik FR
通讯作者：
Jirik FR
影响因子：
3.4
作者：
Herbert, M. R.;Russo, J. P.;Hatchwell, E.
通讯作者：
Hatchwell, E.
DOI：
10.1073/pnas.0605652103
发表时间：
2006-08-29
影响因子：
11.1
作者：
Sitar, Tomasz;Popowicz, Grzegorz M.;Holak, Tad A.
通讯作者：
Holak, Tad A.
影响因子：
3.8
作者：
Bouaziz, Chayma;Martel, Cecile;Bacha, Hassen
通讯作者：
Bacha, Hassen
影响因子：
3.3
作者：
Guo, X;Zuo, H;von der Mark, H
通讯作者：
von der Mark, H