Two-photon Imaging of dendritic spine development in the mouse cortex
Two-photon Imaging of dendritic spine development in the mouse cortex
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DOI：
10.1002/dneu.20630
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发表时间：
2008-05-01
影响因子：
3
通讯作者：
Gan, Wen-Biao
中科院分区：
文献类型：
--
作者：
Pan, Feng;Gan, Wen-Biao
树突棘是哺乳动物大脑中大多数兴奋性突触的突触后部位。随着双光子显微镜和表达荧光蛋白的转基因小鼠的出现，现在可以在几秒到几年的时间尺度内对活的大脑皮层中的树突棘进行成像。最近使用这种体内成像方法的研究已经开始为完整大脑中树突棘的发育和可塑性提供重要的见解。在这里，我们回顾这些研究并讨论图像采集的技术要求。我们预计，个体突触水平的活体双光子成像将极大地扩展我们目前对神经元网络在整个生命过程中如何组装和修改的理解。 (c) 2008 年 Wiley 期刊公司。
Dendritic spines are the postsynaptic sites of most excitatory synapses in the mammalian brain. With the advent of two-photon microscopy and transgenic mice expressing fluorescent proteins, dendritic spines can now be imaged in the living cerebral cortex over time scales ranging from seconds to years. Recent studies with this in vivo imaging approach have begun to provide important insights into the development and plasticity of dendritic spines in the intact brain. Here, we review these studies and discuss technical requirements for image acquisition. We envision that intravital two-photon imaging at the level of individual synapses will greatly expand our current understandings of how neuronal networks are assembled and modified throughout life. (c) 2008 Wiley Periodicals, Inc.