Quantitative parameters of lymphocyte nuclear morphology in bronchoalveolar lavage fluid as novel biomarkers for sarcoidosis.
Quantitative parameters of lymphocyte nuclear morphology in bronchoalveolar lavage fluid as novel biomarkers for sarcoidosis.
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DOI：
10.1186/s13023-021-01926-x
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发表时间：
2021-07-03
影响因子：
3.7
通讯作者：
Hattori N
中科院分区：
文献类型：
--
作者：
Horimasu Y;Yamaguchi K;Sakamoto S;Masuda T;Miyamoto S;Nakashima T;Iwamoto H;Fujitaka K;Hamada H;Hattori N
支气管肺泡灌洗（BAL）是鉴别诊断间质性肺疾病（ILD）的基本检查之一，淋巴细胞增多强烈提示替代诊断而不是特发性肺纤维化。然而，在几种ILD中观察到BALF淋巴细胞增多。我们认为，BALF淋巴细胞核形态的定量评价将有助于鉴别诊断ILD与BALF淋巴细胞分数增加。招募了121例BALF淋巴细胞分数升高的ILD患者（开发队列中68例，验证队列中53例）。在发育队列中，结节病患者的BALF淋巴细胞核显示出比其他ILD患者显著更小的面积、更短的周长、更低的半径比和更大的圆度（每种p < 0.001）。其次，根据受试者工作特征（ROC）分析确定的阈值，结节病患者中具有小面积、短周长、低半径比和圆度增加的淋巴细胞比例高于其他ILD患者（均p < 0.001）。此外，当我们将大小代表参数与形状代表参数相结合时，在发展队列和验证队列中，具有小而圆核的淋巴细胞的分数在区分结节病的ROC曲线下面积约为0.90。本研究首次证明BALF淋巴细胞核形态定量参数作为结节病新生物标志物的有用性。在线版本包含补充材料，可通过10.1186/s13023-021-01926-x获得。
Bronchoalveolar lavage (BAL) is one of the fundamental examinations for the differential diagnosis of interstitial lung diseases (ILDs), and lymphocytosis strongly indicates alternative diagnoses rather than idiopathic pulmonary fibrosis. However, the BALF lymphocytosis is observed in several ILDs. We considered that quantitative evaluation of the BALF lymphocyte nuclear morphology would be useful in the differential diagnosis of ILDs with increased BALF lymphocyte fraction. One hundred and twenty-one patients with ILDs having increased BALF lymphocyte fraction were recruited (68 in the development cohort and 53 in the validation cohort). In the development cohort, BALF lymphocyte nuclei in sarcoidosis patients showed significantly smaller areas, shorter perimeters, lower radius ratios, and increased roundness than those of other ILD patients (p < 0.001 for each). Next, the fractions of lymphocytes with small areas, short perimeters, low radius ratios, and increased roundness, which were determined based on receiver operating characteristic (ROC) analyses-based thresholds, were demonstrated to be higher in sarcoidosis patients than in the other ILD patients (p < 0.001 for each). Furthermore, when we combined size-representing parameters with shape-representing parameters, the fraction of lymphocytes with small and round nuclei showed approximately 0.90 of area under the ROC curve in discriminating sarcoidosis both in the development cohort and the validation cohort. This study is the first to demonstrate the usefulness of quantitative parameters of BALF lymphocyte nuclear morphology as novel biomarkers for sarcoidosis. The online version contains supplementary material available at 10.1186/s13023-021-01926-x.
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DOI：
10.1083/jcb.200912045
发表时间：
2010-06-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Theerthagiri G;Eisenhardt N;Schwarz H;Antonin W
通讯作者：
Antonin W
对医疗统计信息的自由使用的易于使用的软件“ EZR”的调查。
影响因子：
4.8
作者：
Kanda Y
通讯作者：
Kanda Y
DOI：
10.1164/rccm.200504-644oc
发表时间：
2006-01-15
影响因子：
24.7
作者：
Selman, M;Pardo, A;Zlotnik, A
通讯作者：
Zlotnik, A
DOI：
10.1164/rccm.200808-1313oc
发表时间：
2009-06-01
影响因子：
24.7
作者：
Ohshimo, Shinichiro;Bonella, Francesco;Costabel, Ulrich
通讯作者：
Costabel, Ulrich
重塑内质网限制了核包膜形成的速率。
影响因子：
7.8
作者：
Anderson, Daniel J.;Hetzer, Martin W.
通讯作者：
Hetzer, Martin W.