Prognostic value of tumor-infiltrating dendritic cells in colorectal cancer: Role of maturation status and intratumoral localization
Prognostic value of tumor-infiltrating dendritic cells in colorectal cancer: Role of maturation status and intratumoral localization
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DOI：
10.1158/1078-0432.ccr-04-1448
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发表时间：
2005-04-01
影响因子：
11.5
通讯作者：
Kuppen, PJK
中科院分区：
文献类型：
--
作者：
Sandel, MH;Dadabayev, AR;Kuppen, PJK
肿瘤浸润性树突状细胞的临床意义已被报道在各种人类实体瘤中，如肿瘤浸润性树突状细胞的存在与临床预后之间的相关性所示。在这项研究中，我们评估了104例结直肠癌患者原发性肿瘤样本中肿瘤浸润树突状细胞、T淋巴细胞的存在和成熟状态与临床病程之间是否存在相关性。在双重免疫组织化学中用四种不同的标记物（S-100、HILA II类、CD 208和CD 1a）鉴定树突状细胞，层粘连蛋白作为第二标记物以支持精确定位。肿瘤浸润性树突状细胞显示出基于其成熟状态的不同浸润模式。CD 1a阳性树突状细胞大量分布于肿瘤边缘，成熟的CD 208阳性树突状细胞主要分布于肿瘤间质和肿瘤边缘，但在肿瘤上皮中较少见。此外，肿瘤上皮中CD 1a阳性树突状细胞的高浸润与CD 4淋巴细胞的浸润显著相关（P = 0.006）。肿瘤上皮中成熟CD 208阳性浸润树突状细胞数量相对较高的患者总生存期较短（P = 0.004）。此外，在肿瘤进展边缘具有相对较高数量的CD 1a阳性树突状细胞的患者的无病生存期较短（P = 0.03）。我们发现肿瘤浸润性树突状细胞在肿瘤内具有优先浸润位点，影响局部肿瘤细胞-免疫细胞相互作用，并与结直肠癌患者的临床预后相关。
The clinical significance of tumor-infiltrating dendritic cells has been reported in a variety of human solid tumors as shown by the correlations found between the presence of tumor-infiltrating dendritic cells and clinical prognosis. In this study, we evaluated whether there is an association between the presence and maturation status of tumor-infiltrating dendritic cells,T lymphocytes, and clinical course in 104 primary tumor samples of patients with colorectal cancer. Dendritic cells were identified with four different markers (S-100, HILA class II, CD208, and CD1a) in double immunohistochemistry, with laminin as second marker to support the exact localization. Tumor-infiltrating dendritic cells showed a distinct infiltration pattern based on their maturation status. CD1a-positive dendritic cells resided in the advancing tumor margins in relatively high numbers, whereas mature CD208-positive dendritic cells were sparsely present in the tumor epithelium but mainly distributed in the tumor stroma and advancing tumor margin. Furthermore, high infiltration of CD1a-positive dendritic cells in the tumor epithelium was significantly correlated to the infiltration of CD4 lymphocytes (P = 0.006). Patients with relatively high numbers of mature CD208-positive infiltrating dendritic cells in the tumor epithelium had a shorter overall survival (P = 0.004). In addition, patients with relatively high numbers of CD1a-positive dendritic cells in the advancing margin of the tumor had a shorter disease-free survival (P = 0.03). We found that tumor-infiltrating dendritic cells had preferential infiltration sites within a tumor, affected local tumor cell-immune cell interactions, and correlated to the clinical prognosis of colorectal cancer patients.