The Impact of Simulated Weightlessness on Endothelium-Dependent Angiogenesis and the Role of Caveolae/Caveolin-1
The Impact of Simulated Weightlessness on Endothelium-Dependent Angiogenesis and the Role of Caveolae/Caveolin-1
复制标题
模拟失重对内皮依赖性血管生成的影响以及 Caveolae/Caveolin-1 的作用
DOI：
10.1159/000438646
复制
发表时间：
2016-01-01
影响因子：
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通讯作者：
Sun, Xi-Qing
中科院分区：
文献类型：
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作者：
Shi, Fei;Zhao, Tian-Zhi;Sun, Xi-Qing
背景/目的：微重力环境下小窝蛋白-1在调节血管生成中的潜在作用尚未研究。研究方法：利用回转器模拟微重力环境，检测了微重力环境下人脐静脉内皮细胞caveolin-1和eNOS的表达及其相互作用。结果如下：我们发现，小窝蛋白-1的表达减少与微重力刺激的内皮细胞中eNOS的表达和磷酸化水平增加有关，这导致eNOS从小窝蛋白-1复合物中解离。因此，微重力诱导内皮细胞迁移和管腔形成依赖于小窝蛋白-1的调节。结论：我们的研究为改变重力环境中重要的内皮功能提供了见解。
Background/Aims: The potential role of caveolin-1 in modulating angiogenesis in microgravity environment is unexplored. Methods: Using simulated microgravity by clinostat, we measured the expressions and interactions of caveolin-1 and eNOS in human umbilical vein endothelial cells. Results: We found that decreased caveolin-1 expression is associated with increased expression and phosphorylation levels of eNOS in endothelial cells stimulated by microgravity, which causes a dissociation of eNOS from caveolin-1 complexes. As a result, microgravity induces cell migration and tube formation in endothelial cell in vitro that depends on the regulations of caveolin-1. Conclusion: Our study provides insight for the important endothelial functions in altered gravitational environments.