Loop-mediated isothermal amplification (LAMP) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) in China
Loop-mediated isothermal amplification (LAMP) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) in China
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中国水牛（Bubalus babalis, Linnaeus, 1758）中东方巴贝虫的环介导等温扩增（LAMP）检测
DOI：
10.1016/j.vetpar.2009.06.036
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发表时间：
2009-10-28
影响因子：
2.6
通讯作者：
Zhao, Jun-Long
中科院分区：
文献类型：
--
作者：
He, Lan;Zhou, Yan-Qin;Zhao, Jun-Long
环介导等温扩增（LAMP）是一种在等温条件下具有高特异性和高效率的快速方法，使用一组四个特异性设计的引物，其识别靶基因上的六个不同序列。本研究建立了一种特异性检测布法罗（Bubalus babalis，Linnaeus，1758）体内东方Bababellus orientalis的LAMP方法。从B的18S rRNA基因V4高变区设计了4条引物。东方从B采集血样。东方蜱实验感染布法罗和来自湖北省8个不同地区的165头布法罗。提取基因组DNA，进行LAMP测定，并与使用先前描述的半巢式PCR获得的结果进行比较。LAMP检测证明为B。与半巢式PCR相比，而之前的B.在长江以北地区尚未发现东方蜱B. orientalis的报道，因此，B.东方红已经蔓延到了河的北边。这可能会对布法罗产业造成严重威胁。(C)2009 Elsevier B.V.保留所有权利。
Loop-mediated isothermal amplification (LAMP) is a rapid method with high specificity and efficiency under isothermal condition using a set of four specifically designed primers that recognize six distinct sequences on the target gene. In this study, a LAMP method was developed for specific detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758). Four primers were designed from the V4 hypervariable region of the 18S rRNA gene of B. orientalis. Blood samples were collected from B. orientalis experimentally infected water buffalo as well as from 165 water buffalo from eight different regions of the Hubei province, south China. Genomic DNA was extracted, subjected to the LAMP assay and compared with results obtained using a previously described semi-nested PCR. The LAMP assay proofed to be B. orientalis specific and more sensitive than the semi-nested PCR. While previously B. orientalis had not been reported north of the Yangtse River, our results show that B. orientalis has spread to the north of the river. This could pose a serious threat to the water buffalo industry. (C) 2009 Elsevier B.V. All rights reserved.